Nitric Oxide and Parasympathetic Vascular and Secretory Control of the Dog Nasal Mucosa
| Autor: | E K Potter, J S Lacroix, E McLachlan |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Atropine
Male Nitroprusside medicine.medical_specialty Tyrosine 3-Monooxygenase Vasoactive intestinal peptide Blood Pressure Mucous membrane of nose Stimulation Maxillary Artery Nitric Oxide Nitroarginine Parasympathetic nervous system Dogs Internal medicine medicine Animals Neuropeptide Y Trigeminal Nerve Enzyme Inhibitors Trigeminal nerve Dose-Response Relationship Drug Histocytochemistry business.industry NADPH Dehydrogenase General Medicine Propranolol Acetylcholine Electric Stimulation Parasympathetic Fibers Postganglionic Ganglion Nasal Mucosa Endocrinology medicine.anatomical_structure Injections Intra-Arterial Otorhinolaryngology Regional Blood Flow Anesthesia Female business Vasoactive Intestinal Peptide medicine.drug |
| Zdroj: | Acta Oto-Laryngologica. 118:257-263 |
| ISSN: | 1651-2251 0001-6489 |
| Popis: | Nasal vascular and secretory responses to local intra-arterial injection of acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) and to electrical stimulation of the nasal parasympathetic nerve fibres were recorded in dogs anaesthetized with pentobarbital. The influence of pretreatment with atropine and propranolol and the nitric oxide synthetase (NOS) inhibitor Nomega-nitro-L-arginine (L-NNA) was analysed. As a marker for NOS, NADPH-diaphorase (NADPH-d) histochemistry was studied in the sphenopalatine ganglion, trigeminal nerve and nasal mucosa. Local intra-arterial infusion of ACh and VIP evoked dose-dependent vasodilatation and nasal secretion which were not modified in the presence of L-NNA. The NO donor nitroprusside induced dose-dependent vasodilatation but no secretion. Atropine did not reduce the vasodilatation evoked by the parasympathetic nerve stimulation, but did reduce the secretory response by 55% (p < 0.05). During L-NNA infusion, the atropine-resistant vasodilatation evoked by parasympathetic nerve stimulation was reduced by a further 80% (p < 0.01) and the non-cholinergic secretory response was reduced by a further 30% (p < 0.05). Simultaneous infusion of the NO donor nitroprusside reversed the secretory response but not the vasodilator response to parasympathetic nerve stimulation. Histochemical studies revealed that NADPH-d activity was co-localized with VIP in parasympathetic axons. These observations suggest that NO could act as a non-cholinergic parasympathetic neurotransmitter in the vascular and secretory control of the dog nasal mucosa. |
| Databáze: | OpenAIRE |
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