Cyclin E Overexpression Responsible for Growth of Human Hepatic Tumors with p21WAF1/CIP1/SDI1
| Autor: | Masayoshi Namba, Masahiro Miyazaki, Motoaki Ohtsubo, Kazuo Fushimi, Keisuke Hamazaki, Koichiro Mihara, Shouji Furusako, Toshiya Tsuji, Yusuke Inoue, Ryuichiro Ohashi |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Cyclin E Cyclin D Blotting Western Cyclin A Biophysics Cyclin B Protein Serine-Threonine Kinases Retinoblastoma Protein Biochemistry Cyclin D1 Cyclins Proto-Oncogene Proteins CDC2-CDC28 Kinases Tumor Cells Cultured Humans RNA Messenger neoplasms Molecular Biology biology Chemistry Cell Cycle Cyclin-Dependent Kinase 2 Liver Neoplasms Cyclin-dependent kinase 2 Cyclin-Dependent Kinase 4 Cell Biology Cell cycle Blotting Northern Genes p53 Molecular biology Cyclin-Dependent Kinases digestive system diseases Gene Expression Regulation Neoplastic Blotting Southern Kinetics biology.protein biological phenomena cell phenomena and immunity Cyclin A2 |
| Zdroj: | Biochemical and Biophysical Research Communications. 242:317-321 |
| ISSN: | 0006-291X |
| Popis: | We examined a relationship between p21 WAF1/CIP1/SDI1 and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21 WAF1/CIP1/SDI1 protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21 WAF1/CIP1/SDI1 showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21 WAF1/CIP1/SDI1 protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21 WAF1/CIP1/SDI1 -inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E. |
| Databáze: | OpenAIRE |
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