Apolipoprotein A-I Exerts Bactericidal Activity against Yersinia enterocolitica Serotype O:3
| Autor: | T. Sakari Jokiranta, Matti Jauhiainen, Artur Muszyński, Taru Meri, Vassilis I. Zannis, Joanna Radziejewska-Lebrecht, Marta Biedzka-Sarek, Jari Metso, Andreas Kateifides, Mikael Skurnik |
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| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Apolipoprotein B Surface Properties Microbiology Biochemistry 03 medical and health sciences chemistry.chemical_compound polycyclic compounds Humans Yersinia enterocolitica Molecular Biology Pathogen 030304 developmental biology Antigens Bacterial 0303 health sciences Binding Sites Innate immune system Apolipoprotein A-I biology 030306 microbiology Effector Temperature O Antigens nutritional and metabolic diseases Complement System Proteins Cell Biology biology.organism_classification Blood proteins Immunity Innate Anti-Bacterial Agents 3. Good health chemistry Mutation biology.protein lipids (amino acids peptides and proteins) Lipoproteins HDL Peptides Bacteria |
| Zdroj: | Journal of Biological Chemistry. 286:38211-38219 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m111.249482 |
| Popis: | Apolipoprotein A-I (apoA-I), the main protein component of high density lipoprotein (HDL), is well recognized for its antiatherogenic, antioxidant, and antiinflammatory properties. Here, we report a novel role for apoA-I as a host defense molecule that contributes to the complement-mediated killing of an important gastrointestinal pathogen, Gram-negative bacterium Yersinia enterocolitica. We specifically show that the C-terminal domain of apoA-I is the effector site providing the bactericidal activity. Although the presence of the lipopolysaccharide O-antigen on the bacterial surface is absolutely required for apoA-I to kill the bacteria, apoA-I does not interact with the bacteria directly. To the contrary, exposure of the bacteria by serum proteins triggers apoA-I deposition on the bacterial surface. As our data show that both purified lipid-free and HDL-associated apoA-I displays anti-bacterial potential, apoA-I mimetic peptides may be a promising therapeutic agent for the treatment of certain Gram-negative infections. |
| Databáze: | OpenAIRE |
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