The chromatin-binding protein PHF6 functions as an E3 ubiquitin ligase of H2BK120 via H2BK12Ac recognition for activation of trophectodermal genes
| Autor: | Jaebeom Kim, Yoon Kyung Jeon, Ho Lee, Ji Min Lee, Hee Jung Choi, Seon Ah Baek, Kyungjin Boo, Sung Hee Baek, Daechan Park, Sungryong Oh, Junghyun You |
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| Rok vydání: | 2020 |
| Předmět: |
AcademicSubjects/SCI00010
Ubiquitin-Protein Ligases Histones Mice 03 medical and health sciences 0302 clinical medicine Ubiquitin Gene expression Genetics Animals Humans Epigenetics 030304 developmental biology Homeodomain Proteins 0303 health sciences biology Chromatin binding Gene regulation Chromatin and Epigenetics Ubiquitination Acetylation Mouse Embryonic Stem Cells Cellular Reprogramming Chromatin Cell biology Ubiquitin ligase Repressor Proteins Histone 030220 oncology & carcinogenesis embryonic structures biology.protein Homeobox Protein Processing Post-Translational Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkaa626 |
| Popis: | Epigenetic regulation is important for establishing lineage-specific gene expression during early development. Although signaling pathways have been well-studied for regulation of trophectoderm reprogramming, epigenetic regulation of trophectodermal genes with histone modification dynamics have been poorly understood. Here, we identify that plant homeodomain finger protein 6 (PHF6) is a key epigenetic regulator for activation of trophectodermal genes using RNA-sequencing and ChIP assays. PHF6 acts as an E3 ubiquitin ligase for ubiquitination of H2BK120 (H2BK120ub) via its extended plant homeodomain 1 (PHD1), while the extended PHD2 of PHF6 recognizes acetylation of H2BK12 (H2BK12Ac). Intriguingly, the recognition of H2BK12Ac by PHF6 is important for exerting its E3 ubiquitin ligase activity for H2BK120ub. Together, our data provide evidence that PHF6 is crucial for epigenetic regulation of trophectodermal gene expression by linking H2BK12Ac to H2BK120ub modification. |
| Databáze: | OpenAIRE |
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