Relationship between age and blastocyst chromosomal ploidy analyzed by noninvasive preimplantation genetic testing for aneuploidies (niPGT-A)
| Autor: | Felipe Dieamant, Jonathas B. Soares, Alvaro P. Ceschin, Raul Nakano, Condesmar Marcondes, Claudia G. Petersen, Antônio Carlos Costa Franco, Adelino Amaral, Laura D. Vagnini, Antonio Hélio Oliani, Carlos Gilberto Almodin, Jose G. Franco, Joaquim Roberto Costa Lopes, Joao Batista A. Oliveira, Maria do Carmo Tomitão Canas, Adriana Renzi |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Monosomy Aneuploidy Biology Preimplantation genetic diagnosis Embryo Culture Techniques Andrology Young Adult Pregnancy medicine Humans niPGT-A Genetic Testing Blastocyst Preimplantation Diagnosis preimplantation genetic diagnosis Mosaicism Age Factors MALBAC High-Throughput Nucleotide Sequencing Karyotype free DNA medicine.disease medicine.anatomical_structure age Female Original Article Klinefelter syndrome Trisomy PGT-A |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 |
| Popis: | Objective To assess the relationship between human blastocyst chromosomal ploidy established by niPGT-A and increasing age. Methods This is a prospective multicenter study carried out by ten assisted reproduction centers after their embryologists acquired training and validated their results with the previous use of niPGT-A. A total of 94 couples with indication for niPGT-A due to increase maternal age, male factor, repeated implantation failures, recurrent abortion or because they requested niPGT-A were included in this study. The couples had no karyotype abnormalities. After ICSI, the embryos were cultured until blastocyst stage using one or two step culture systems, single or sequential media respectively, at 37°C in an atmosphere of 6-7% CO2 and 5-20% O2 incubators. On day 3, we re-evaluated cleavage embryos to complete cumulus cells removal. The embryos were then cultured in individual well, with 20µl of medium under oil until they reached blastocyst stage. The blastocysts were vitrified and stored in liquid nitrogen. After that, the spent blastocyst culture medium (20µl) was transferred to a PCR tube and sent for analysis in the genetic laboratory, where it was stored at -80°C until sequencing. A total of 243 samples of spent blastocyst culture medium were collected on the 5th/6th day. Cell-free DNA secreted on culture medium was amplified using NICS Sample Preparation Kit (Yikon Genomics), based on the MALBAC technology. After whole genome amplification, the DNA was measured using a Qubit 2.0 fluorometer and subjected to next generation sequencing (NGS) using Illumina MiSeq® platform. The data were analyzed using the ChromGo® software (Yikon Genomics). Results The mean age of the patients was 38±4.08 years with an interval of 20-44 years. The euploid was diagnosed in 36.4% (80/220) of cases, aneuploidy in 31.3% (69/220), and mosaicism in 32.3% (71/220; with ≥60% aneuploidy) of blastocysts. Mosaic values ranged from 29.8% to 33.8% in different age groups. Individually, the most frequent chromosomal abnormality was XXY (Klinefelter Syndrome) occurring in 18 cases, followed by chromosome 21 (trisomy/monosomy) in 8 cases. The niPGT-A data showed a ≥60% incidence of aneuploid cells in all cases of chromosomal mosaicism (n=71). Conclusion A high degree of mosaicism with aneuploidy cells was detected, and some hypotheses were suggested for this data (niPGT-A sensitivity in detecting the self-correction of chromosomal abnormalities phenomenon). However, it did not vary remarkably with age. On the other hand, euploidy levels had a negative correlation with age and aneuploidy levels had a positive relationship. This is the first report in the literature to relate chromosomal ploidy in blastocysts using niPGT-A and increasing patient age. |
| Databáze: | OpenAIRE |
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