Synthesis and characterization of low molecular weight polyethyleneimine-terminated Poly(β-amino ester) for highly efficient gene delivery of minicircle DNA
| Autor: | Jing Zhao, Zhiyong Wang, Cheng-Yi He, Jiajia Pan, Zhi-Ying Chen, Ping Huang, Hong Liu, Yan Tan, Yang Lei |
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| Rok vydání: | 2016 |
| Předmět: |
Polymers
Surface Properties Swine Genetic enhancement 02 engineering and technology Gene delivery 010402 general chemistry 01 natural sciences Cell Line Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Animals Humans Polyethyleneimine Particle Size Cytotoxicity chemistry.chemical_classification Polyethylenimine Chemistry Gene Transfer Techniques Polymer Transfection 021001 nanoscience & nanotechnology 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Molecular Weight Biochemistry Cell culture Reagent Nanoparticles DNA Circular 0210 nano-technology |
| Zdroj: | Journal of Colloid and Interface Science. 463:93-98 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/j.jcis.2015.10.025 |
| Popis: | Gene therapy has held great promise for treating specific acquired and inherited diseases. However, the lack of safe and efficient gene delivery systems remains as the major challenge. Poly(β-amino ester)s (PBAEs) have attracted much attention due to their outstanding properties in biosafety, DNA delivery efficiency and convenience in synthesis. In this paper, we reported the further enhancement of the PBAE functions by increasing its positive charge through conjugating with low molecular weight polyethylenimine (LPEI). The resulted LPEI-PBAE polymer was able to condense minicircle DNA (mcDNA) forming nanoparticles with a diameter of 50-200nm. Furthermore, as compared to parental PBAE and a commercial transfection reagent very common in laboratory application, the LPEI-PBAE demonstrated significantly higher transfection efficiency with little cytotoxicity. These results suggested LPEI-PBAEs are worthy of further optimization for gene therapy applications. |
| Databáze: | OpenAIRE |
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