Increased T-cell responses to Epstein–Barr virus with high viral load in patients with Epstein–Barr virus-positive diffuse large B-cell lymphoma

Autor: Yasuo Morishima, Hidetsugu Mihara, Keitaro Tsushita, Kazuhito Yamamoto, Hiroshi Kimura, Kiyotaka Kuzushima, Yoshitoyo Kagami, Satoko Morishima, Masafumi Ito, Nobuhiko Emi, Suzuko Moritani, Hirokazu Nagai, Hiroshi Onoda, K Miyamura, Shigeo Nakamura, Tomohiro Kinoshita, Hidemasa Miyauchi, Yasushi Yatabe, Sachiko Iba, Masataka Okamoto, Seiko Iwata, Kaneyuki Ohbayashi, Isamu Sugiura
Rok vydání: 2014
Předmět:
Zdroj: Leukemia & Lymphoma. 56:1072-1078
ISSN: 1029-2403
1042-8194
DOI: 10.3109/10428194.2014.938326
Popis: The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.
Databáze: OpenAIRE