Increased T-cell responses to Epstein–Barr virus with high viral load in patients with Epstein–Barr virus-positive diffuse large B-cell lymphoma
| Autor: | Yasuo Morishima, Hidetsugu Mihara, Keitaro Tsushita, Kazuhito Yamamoto, Hiroshi Kimura, Kiyotaka Kuzushima, Yoshitoyo Kagami, Satoko Morishima, Masafumi Ito, Nobuhiko Emi, Suzuko Moritani, Hirokazu Nagai, Hiroshi Onoda, K Miyamura, Shigeo Nakamura, Tomohiro Kinoshita, Hidemasa Miyauchi, Yasushi Yatabe, Sachiko Iba, Masataka Okamoto, Seiko Iwata, Kaneyuki Ohbayashi, Isamu Sugiura |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Epstein-Barr Virus Infections Herpesvirus 4 Human Cancer Research T-Lymphocytes T cell CD8-Positive T-Lymphocytes Biology Antibodies Viral medicine.disease_cause Interferon-gamma Immune system immune system diseases hemic and lymphatic diseases medicine Humans Lymphocyte Count Prospective Studies Cells Cultured Immunodeficiency Aged Cell Proliferation Aged 80 and over B-Lymphocytes Epstein-Barr Virus Positive Hematology Middle Aged Viral Load Flow Cytometry medicine.disease Virology Epstein–Barr virus Lymphoma medicine.anatomical_structure Oncology Immunoglobulin G DNA Viral Host-Pathogen Interactions Immunology Female Lymphoma Large B-Cell Diffuse Diffuse large B-cell lymphoma Viral load |
| Zdroj: | Leukemia & Lymphoma. 56:1072-1078 |
| ISSN: | 1029-2403 1042-8194 |
| Popis: | The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL. |
| Databáze: | OpenAIRE |
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