Native whey protein with high levels of leucine results in similar post-exercise muscular anabolic responses as regular whey protein: A randomized controlled trial
| Autor: | Gøran Paulsen, Sigve Nyvik Aas, Ina Garthe, Truls Raastad, Elisabet Børsheim, Kristin Tosterud Holte, Haakon B. Benestad, Håvard Hamarsland, Anne Lene Nordengen, Matthew Cotter |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine protein quality medicine.medical_specialty Whey protein Anabolism Muscle Proteins stable isotopes lcsh:TX341-641 Clinical nutrition Biology amion acids Young Adult 03 medical and health sciences fluids and secretions Double-Blind Method Leucine Internal medicine medicine Humans Ingestion lcsh:Sports medicine skeletal muscle Muscle Skeletal Cross-Over Studies 030109 nutrition & dietetics Nutrition and Dietetics food and beverages Skeletal muscle Crossover study Healthy Volunteers Sports Nutritional Physiological Phenomena Whey Proteins Endocrinology medicine.anatomical_structure nutrition Biochemistry Protein Biosynthesis Dietary Supplements supplementation Amino acids Female resistance training lcsh:RC1200-1245 lcsh:Nutrition. Foods and food supply Protein quality Research Article Food Science |
| Zdroj: | Journal of the International Society of Sports Nutrition Journal of the International Society of Sports Nutrition, Vol 14, Iss 1, Pp 1-12 (2017) |
| Popis: | Background Protein intake is essential to maximally stimulate muscle protein synthesis, and the amino acid leucine seems to possess a superior effect on muscle protein synthesis compared to other amino acids. Native whey has higher leucine content and thus a potentially greater anabolic effect on muscle than regular whey (WPC-80). This study compared the acute anabolic effects of ingesting 2 × 20 g of native whey protein, WPC-80 or milk protein after a resistance exercise session. Methods A total of 24 young resistance trained men and women took part in this double blind, randomized, partial crossover, controlled study. Participants received either WPC-80 and native whey (n = 10), in a crossover design, or milk (n = 12). Supplements were ingested immediately (20 g) and two hours after (20 g) a bout of heavy-load lower body resistance exercise. Blood samples and muscle biopsies were collected to measure plasma concentrations of amino acids by gas-chromatography mass spectrometry, muscle phosphorylation of p70S6K, 4E–BP1 and eEF-2 by immunoblotting, and mixed muscle protein synthesis by use of [2H5]phenylalanine-infusion, gas-chromatography mass spectrometry and isotope-ratio mass spectrometry. Being the main comparison, differences between native whey and WPC-80 were analysed by a one-way ANOVA and comparisons between the whey supplements and milk were analysed by a two-way ANOVA. Results Native whey increased blood leucine concentrations more than WPC-80 and milk (P |
| Databáze: | OpenAIRE |
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