A Multicenter Trial of the Effectiveness of ζ-Globin Enzyme-Linked Immunosorbent Assay and Hemoglobin H Inclusion Body Screening for the Detection of α0-Thalassemia Trait
| Autor: | Mark Crowther, John D. Lafferty, Andrew McFarlane, Linda M. Halchuk, Anne Raby, David Barth, Brian L. Sheridan |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Hemolytic anemia Adolescent Thalassemia Enzyme-Linked Immunosorbent Assay Southeast asian Polymerase Chain Reaction Hemoglobin Barts alpha-Thalassemia hemic and lymphatic diseases Hydrops fetalis medicine Humans Genetic Testing Globin Hemoglobin H Disease Hemoglobin H business.industry Genetic Carrier Screening General Medicine medicine.disease Virology Globins Erythrocyte Inclusions Hemoglobinopathy Immunology business |
| Zdroj: | American Journal of Clinical Pathology. 129:309-315 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1309/mnpf3xxxvax5nm9h |
| Popis: | Routine laboratories use a hemoglobin H (HbH) screen to detect alpha-thalassemia carriers of fatal hemoglobin Bart's hydrops fetalis. This test is laborious and has sensitivity concerns. A commercial zeta-globin enzyme-linked immunosorbent assay (ELISA) is effective in detecting Southeast Asian (SEA) alpha-thalassemia. We present results of a study of the effectiveness of carrier detection of ELISA and a shortened HbH screen compared with gap polymerase chain reaction. ELISA was superior to the HbH screen for the SEA alpha0-thalassemia trait. The ELISA and H screen were equal for detection of all carriers encountered and combined were more effective than either test alone. A positive zeta-globin ELISA result is diagnostic of SEA alpha-thalassemia, and routine use of the zeta-globin ELISA in combination with a shortened HbH screen will improve the efficacy of prenatal screening for carriers of hemoglobin Bart's hydrops fetalis through improved detection and referral for follow-up DNA testing. |
| Databáze: | OpenAIRE |
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