Cryopreservation of Rat Leydig Cells For In Vitro and In Vivo Studies
| Autor: | Tze Wj, Tai J, Johnson Hw |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
endocrine system medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases Cryobiology Endocrinology Diabetes and Metabolism Clinical Biochemistry Biology Testicle Kidney Biochemistry Cryopreservation Andrology Endocrinology In vivo Internal medicine medicine Animals Dimethyl Sulfoxide Testosterone Secretion Rats Wistar Cells Cultured Leydig cell urogenital system Biochemistry (medical) Leydig Cells General Medicine In vitro Rats medicine.anatomical_structure Rats Inbred Lew |
| Zdroj: | Hormone and Metabolic Research. 26:145-147 |
| ISSN: | 1439-4286 0018-5043 |
| Popis: | Leydig cells lose their ability to secrete testosterone following short-term in vitro culture. A procedure for effective storage of these cells would be useful. In this study, rat Leydig cells were cryopreserved in the presence of 10, 15 or 20% dimethylsulfoxide (DMSO) at approximately 1 degree C/min to -70 degrees C and then stored in LN2. After thawing, the cells cryopreserved in the presence of 15% DMSO showed the highest viability of over 75%. These cells secreted basal levels of testosterone in vitro as well as responded to hCG stimulation by secreting over 9-fold increase in testosterone. The viability of these cells was further confirmed by the demonstration of 3 beta-HSD positive cells under the kidney capsule of rats isografted with cryopreserved Leydig cells. This study demonstrated that purified rat Leydig cells can be cryopreserved and the cryopreserved cells retained normal function and were responsive to hCG stimulation. Cryopreservation is a simple procedure for long-term storage of functional Leydig cells. |
| Databáze: | OpenAIRE |
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