Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats
| Autor: | Caroline Barbé, Jérôme Salles, Christophe Chambon, Christophe Giraudet, Phelipe Sanchez, Véronique Patrac, Philippe Denis, Yves Boirie, Stéphane Walrand, Marine Gueugneau |
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| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Proteomics proteome [SDV]Life Sciences [q-bio] Organic Chemistry Malnutrition aging General Medicine skeletal muscle sarcopenia undernutrition Catalysis Computer Science Applications Rats Inorganic Chemistry Animals Physical and Theoretical Chemistry Muscle Skeletal Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2022, 23 (9), ⟨10.3390/ijms23094762⟩ International Journal of Molecular Sciences; Volume 23; Issue 9; Pages: 4762 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms23094762⟩ |
| Popis: | Aging is associated with a progressive loss of skeletal muscle mass and function termed sarcopenia. Various metabolic alterations that occur with aging also increase the risk of undernutrition, which can worsen age-related sarcopenia. However, the impact of undernutrition on aged skeletal muscle remains largely under-researched. To build a deeper understanding of the cellular and molecular mechanisms underlying age-related sarcopenia, we characterized the undernutrition-induced changes in the skeletal muscle proteome in old rats. For this study, 20-month-old male rats were fed 50% or 100% of their spontaneous intake for 12 weeks, and proteomic analysis was performed on both slow- and fast-twitch muscles. Proteomic profiling of undernourished aged skeletal muscle revealed that undernutrition has profound effects on muscle proteome independently of its effect on muscle mass. Undernutrition-induced changes in muscle proteome appear to be muscle-type-specific: slow-twitch muscle showed a broad pattern of differential expression in proteins important for energy metabolism, whereas fast-twitch muscle mainly showed changes in protein turnover between undernourished and control rats. This first proteomic analysis of undernourished aged skeletal muscle provides new molecular-level insight to explain phenotypic changes in undernourished aged muscle. We anticipate this work as a starting point to define new biomarkers associated with undernutrition-induced muscle loss in the elderly. |
| Databáze: | OpenAIRE |
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