In vitro assessment of the bioavailability of bioactive non-extractable polyphenols obtained by pressurized liquid extraction combined with enzymatic-assisted extraction from sweet cherry (Prunus avium L.) pomace
| Autor: | Domínguez Rodríguez, Gloria, Marina Alegre, María Luisa, Plaza del Moral, Merichel |
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| Přispěvatelé: | Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química |
| Rok vydání: | 2022 |
| Předmět: |
Bioavailability
Plant Extracts Sweet cherry pomace Biological Availability Polyphenols General Medicine Química Prunus avium Antioxidants Analytical Chemistry Chemistry Phenols Pressurized liquid extraction Non-extractable polyphenols Chromatography High Pressure Liquid Enzyme-assisted extraction Food Science |
| Zdroj: | e_Buah Biblioteca Digital Universidad de Alcalá Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| Popis: | In vitro digestion and absorption simulation processes of non-extractable polyphenols (NEPs) obtained by pressurized liquid extraction combined with enzymatic-assisted extraction with Promod enzyme (PLE-EAE) from the residue of conventional extraction of sweet cherry pomace were studied. In general, total phenolic and proanthocyanidin contents decreased in each phase of the digestion. However, the antioxidant capacity increased when the digestion process progressed. In addition, the highest total phenolic and proanthocyanidin contents and antioxidant capacity were obtained in the absorbed fraction. NEPs from PLE-EAE extract, digestive fractions, absorbed and unabsorbed fractions were analyzed by ultra-high-performance liquid chromatography coupled to electrospray ionization quadrupole Exactive-Orbitrap mass spectrometry (UHPLC-ESI-Q-Orbitrap-MS). Fifteen NEPs were identified in the intestinal fraction and five in the absorbed fraction after the digestion process. Results obtained in this study define for the first time the bioavailability of antioxidant NEPs obtained from sweet cherry pomace. |
| Databáze: | OpenAIRE |
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