A flotation/sieving method to detect Echinococcus multilocularis and Toxocara spp. eggs in soil by real-time PCR
| Autor: | Franck Boué, Jean-Marc Boucher, Camille Renault, Marie-Lazarine Poulle, Gérald Umhang, Christophe Caillot, Matthieu Bastien, Vanessa Hormaz, Marine Faisse |
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| Přispěvatelé: | Protozooses Transmises par l'Alimentation (Cryptosporidiose, Giardose et Toxoplasmose) : Mode de Contamination et Pathogénie (PROTAL) - EA 3800 (PROTAL), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA), Centre de Recherche et de Formation en Eco-éthologie (2C2A-CERFE), Université de Reims Champagne-Ardenne (URCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Carnivora Foxes Toxocara spp Feces Soil Soil contamination [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Environmental samples Zoonoses Parasite hosting ComputingMilieux_MISCELLANEOUS biology 030108 mycology & parasitology DNA Helminth Echinococcosis 3. Good health Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Research Article Veterinary (miscellaneous) Echinococcus multilocularis Real-Time Polymerase Chain Reaction Sensitivity and Specificity lcsh:Infectious and parasitic diseases Toxascaris 03 medical and health sciences Dogs parasitic diseases medicine Helminths Animals lcsh:RC109-216 Ovum Toxocara [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health biology.organism_classification medicine.disease DNA extraction Insect Science Cats Animal Science and Zoology Parasitology Parasite eggs Real-time PCR |
| Zdroj: | Parasite, Vol 24, p 28 (2017) Parasite Parasite, EDP Sciences, 2017, 24, pp.28. ⟨10.1051/parasite/2017029⟩ |
| ISSN: | 1776-1042 1252-607X |
| Popis: | Soil can be a source of human infection by many zoonotic helminth species including Echinococcus multilocularis and Toxocara spp. The prevention of alveolar echinococcosis could be greatly improved through the identification of at-risk areas. Yet very few data are available about the detection of E. multilocularis in soil, while more studies have been reported for Toxocara spp. Identification of soil contamination by E. multilocularis eggs requires the use of specific methods. This study describes the development of a method for the detection of E. multilocularis in soil samples with the concentration of eggs using a flotation/sieving method and detection by duplex real-time polymerase chain reaction (PCR). Toxocara spp. egg detection was also undertaken due to the widespread presence of this parasite in soil, despite it being considered less pathogenic. Method sensitivity of 100% was reached for the detection of 10 E. multilocularis eggs spiked in 10 g of soil. Concerning Toxocara spp., method sensitivity was lower but assumed to be due to the reduced effectiveness of the DNA extraction protocol. The parasitological status for E. multilocularis and Toxocara spp. of 63 carnivore fecal samples collected in highly endemic rural areas of France and of soil samples collected under and near these fecal samples was compared. The contamination of soil samples collected under positive fecal samples for E. multilocularis (n = 3) or Toxocara spp. (n = 19) confirmed the transfer of eggs from the definitive host to the environment. |
| Databáze: | OpenAIRE |
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