Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues

Autor: Giorgia Del Vecchio, Rodolfo Negri, Roberto Amendola, Valerio Licursi, Cecilia Mannironi, Carlo Presutti, Mariangela Cestelli Guidi
Rok vydání: 2017
Předmět:
0301 basic medicine
Leptin
Pathology
medicine.medical_specialty
Cells
education
Peptide hormone
Biology
Radiation Dosage
ionizing radiation
miRNA
radiotherapy
transcriptional response to irradiation
Animals
Cells
Cultured
Dose-Response Relationship
Drug
Epithelial Cells
Humans
Mice
Transcriptome
Radiological and Ultrasound Technology
Radiology
Nuclear Medicine and Imaging
Dose-Response Relationship
03 medical and health sciences
0302 clinical medicine
Nuclear Medicine and Imaging
microRNA
medicine
Radiology
Nuclear Medicine and imaging
health care economics and organizations
Messenger RNA
Cultured
Epidermis (botany)
Microarray analysis techniques
Cancer
medicine.disease
Cell biology
030104 developmental biology
030220 oncology & carcinogenesis
Drug
Radiology
hormones
hormone substitutes
and hormone antagonists
Zdroj: International journal of radiation biology
(2017): 947–957. doi:10.1080/09553002.2017.1339918
info:cnr-pdr/source/autori:Licursi V.; Cestelli Guidi M.; Del Vecchio G.; Mannironi C.; Presutti C.; Amendola R.; Negri R./titolo:Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues/doi:10.1080%2F09553002.2017.1339918/rivista:International journal of radiation biology (Print)/anno:2017/pagina_da:947/pagina_a:957/intervallo_pagine:947–957/volume
ISSN: 1362-3095
Popis: Purpose: Leptin (LEP) is a peptide hormone with multiple physiological functions. Besides its systemic actions, it has important peripheral roles such as a mitogen action on keratinocytes following skin lesions. We previously showed that LEP mRNA is significantly induced in response to neutron irradiation in mouse skin and that the protein increases in the irradiated epidermis and in the related subcutaneous adipose tissue. In this work, we investigated the post-transcriptional regulation of LEP by miRNAs and the conservation of LEP's role in radiation response in human cells. Methods: We used microarray analysis and real-time polymerase chain reaction (RT-PCR) to analyze modulation of miRNAs potentially targeting LEP in mouse skin following irradiation and bioinformatic analysis of transcriptome of irradiated human cell lines and cancer tissues from radiotherapy-treated patients to evaluate LEP expression. Results and conclusions: We show that a network of miRNAs potentially targeting LEP mRNA is modulated in irradiated mouse skin and that LEP itself is significantly modulated by irradiation in human epithelial cell lines and in breast cancer tissues from radiotherapy-treated patients. These results confirm and extend the previous evidence that LEP has a general and important role in the response of mammalian cells to irradiation.
Databáze: OpenAIRE
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