A new continuous system of enzymatic hydrolysis coupled with membrane separation for isolation of peptides with angiotensin I converting enzyme inhibitory capacity from defatted corn germ protein
| Autor: | Yuqing Duan, Abubakr Musa, Mohammed A. A. Gasmalla, Haile Ma, Emad Karrar, Asif Wali, Frederick Sarpong |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Membranes Chromatography Protein Hydrolysates Chemistry Hydrolysis Substrate (chemistry) Angiotensin-Converting Enzyme Inhibitors Peptide General Medicine Chemical Fractionation Zea mays Hydrolysate Membrane technology Membrane Enzyme Ultrasonic Waves Enzymatic hydrolysis Peptides IC50 Plant Proteins Food Science |
| Zdroj: | Food & Function. 11:1146-1154 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c9fo01980d |
| Popis: | In this study, separation of peptides with Angiotensin I converting enzyme (ACE)-inhibitory capacity obtained from ultrasonically pretreated defatted corn germ protein (DCGP) by using a new continuous system of enzymatic hydrolysis coupled with membrane separation (EHC-MS) was investigated. Ultrasonic pretreatment was applied to enhance the enzymatic hydrolysis rate of DCGP, as proved in our previous study. The EHC-MS system was operated in two modes which included the batch system and continuous system with continuous water and substrate feeding and was compared with the EH-offline-MS system. The selection of the membrane was based on the hydrolysate fraction which had the highest activity for inhibition of ACE. The results showed that the 1-3 kDa fraction of DCGP hydrolysates had the lowest IC50 value (0.124 mg mL-1) for inhibition of ACE. The degree of conversion (%) of DCGP and output of peptides per unit of the enzyme were significantly (P < 0.05) increased by 55.3% and 55% in the EHC-MS batch process and 79% and 473% in the EHC-MS continuous operation compared to the EH-offline-MS system. The EHC-MS using continuous water and substrate feeding operation was noted to be the best in terms of a high degree of DCG protein conversion (75.68 ± 1.34) and the output of peptides per unit of the enzyme (78.65 ± 1.13). The results revealed that the EHC-MS method with constant water and substrate feeding could show a better application in peptide production in the food industry. |
| Databáze: | OpenAIRE |
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