Culicoides antigen extract stimulates equine blood mononuclear (BMN) cell proliferation and the release of eosinophil adherence-inducing factor(s)
| Autor: | J. McKelvie, Fiona M. Cunningham, A.S. Hamblin, A. P. Foster |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Hot Temperature
CD3 Complex Stimulation Corynebacterium Ceratopogonidae Lymphocyte Activation Adjuvants Immunologic Antigen Cell Adhesion Tetanus Toxoid medicine Animals Horses Intradermal injection Antigens Phytohemagglutinins Phytohaemagglutinin General Veterinary biology business.industry Pruritus Toxoid Antibodies Monoclonal T lymphocyte Eosinophil Recombinant Proteins Eosinophils Sweet itch medicine.anatomical_structure Immunology Leukocytes Mononuclear biology.protein Horse Diseases Interleukin-5 business Cell Division |
| Zdroj: | Research in Veterinary Science. 70:115-122 |
| ISSN: | 0034-5288 |
| DOI: | 10.1053/rvsc.2000.0452 |
| Popis: | Intradermal injection of a Culicoides antigen extract (CAgX) induces T lymphocyte and eosinophil accumulation in the skin of horses with sweet itch. Blood mononuclear ( BMN ) cells from normal ponies proliferate when stimulated by mitogen (phytohaemagglutinin, PHA ) or antigen (tetanus toxoid, TT ) and, as shown here, release soluble factor(s) that induce eosinophil adherence. CAgX also caused concentration dependent proliferation of BMN cells from sweet itch and normal ponies [stimulation index: 29 (13) and 17 (7) for BMN cells from sweet itch and normal ponies, respectively during the active phase of disease; 4 μg protein ml−1CAgX; 168 h]. A heat labile factor(s) which caused eosinophil adherence was also released [sweet itch ponies: 6.0 (1.6) per cent adherence versus 1.3 (0.4) per cent; normal ponies: 6.6 (0.5) per cent adherence versus 0.9 (0.1) per cent for supernatants from CAgX (4 μg protein ml−1; 48 hours) stimulated versus unstimulated BMN cells, respectively]. These results suggest that soluble proteins released from T lymphocytes could affect eosinophil function in the lesional skin of sweet itch horses. |
| Databáze: | OpenAIRE |
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