Lithocholic acid-sensitive mutant cells isolated from Chinese hamster ovary cells are cross-sensitive to cytoskeleton-disturbing agents
| Autor: | Kaneko Motohisa, Kodama Masahiko, Horikoshi Junko, Inoue Fumiko |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lithocholic acid
Paclitaxel medicine.drug_class Cell Survival Cytochalasin B Mutant CHO Cells Biology Toxicology Colony-Forming Units Assay chemistry.chemical_compound Cricetinae medicine Colchicine Animals Cytoskeleton Bile acid Dose-Response Relationship Drug Chinese hamster ovary cell Deoxycholic acid Cholic acid Demecolcine General Medicine chemistry Biochemistry Cell culture Mutagenesis Ethyl Methanesulfonate Mutation Lithocholic Acid |
| Zdroj: | Toxicology letters. 66(3) |
| ISSN: | 0378-4274 |
| Popis: | The lithocholic acid-sensitive mutant cells were isolated from CHO-9 cells mutagenized with ethylmethane sulfonate, through clonal selection on 96-well dishes. The isolated mutant cells (LSJ-11) were 3-fold more sensitive than controls in the survival to lithocholic acid and were also sensitive to other bile acids such as deoxycholic acid and cholic acid. As a preliminary characterization, it was found that the mutant cells were also sensitive to cytoskeleton-disturbing agents such as colchicine, colcemide, taxol and cytochalacin B. This is the first observation that a bile acid-sensitive mutant was isolated and was partially characterized. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|