Calcium-Lipid Interactions Observed with Isotope-Edited Infrared Spectroscopy
| Autor: | Ron Elber, Mason L. Valentine, Alfredo E. Cardenas, Carlos R. Baiz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Lipid Bilayers
Biophysics Infrared spectroscopy chemistry.chemical_element Calcium Coordination complex 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Isotopes Phosphatidylcholine Lipid bilayer 030304 developmental biology chemistry.chemical_classification 0303 health sciences Hydrogen bond Hydrogen Bonding Phosphatidylserine Articles Membrane chemistry Phosphatidylcholines lipids (amino acids peptides and proteins) 030217 neurology & neurosurgery |
| Zdroj: | Biophys J |
| Popis: | Calcium ions bind to lipid membranes containing anionic lipids; however, characterizing the specific ion-lipid interactions in multicomponent membranes has remained challenging because it requires nonperturbative lipid-specific probes. Here, using a combination of isotope-edited infrared spectroscopy and molecular dynamics simulations, we characterize the effects of a physiologically relevant (2 mM) Ca2+ concentration on zwitterionic phosphatidylcholine and anionic phosphatidylserine lipids in mixed lipid membranes. We show that Ca2+ alters hydrogen bonding between water and lipid headgroups by forming a coordination complex involving the lipid headgroups and water. These interactions distort interfacial water orientations and prevent hydrogen bonding with lipid ester carbonyls. We demonstrate, experimentally, that these effects are more pronounced for the anionic phosphatidylserine lipids than for zwitterionic phosphatidylcholine lipids in the same membrane. |
| Databáze: | OpenAIRE |
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