Widespread dispersal of cholera toxin subunit b to brain and spinal cord neurons following systemic delivery
| Autor: | Beverly L. Davidson, Joseph Martin Alisky, Christopher I. van de Wetering |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cholera Toxin medicine.disease_cause Injections Intramuscular Central nervous system disease Mice Developmental Neuroscience Adjuvants Immunologic medicine Animals Neurons Afferent Amyotrophic lateral sclerosis Motor Neurons business.industry Area postrema Cholera toxin Brain Motor neuron Spinal cord medicine.disease Sensory neuron Mice Inbred C57BL medicine.anatomical_structure Neurology Spinal Cord Injections Intravenous Axoplasmic transport business Neuroscience Injections Intraperitoneal |
| Zdroj: | Experimental neurology. 178(1) |
| ISSN: | 0014-4886 |
| Popis: | We have discovered novel transport properties of cholera toxin subunit b beyond well-known anterograde and retrograde axonal transport. Injection of 1500 microg of CTb intraperitoneally or intravenously in young adult mice resulted in generalized enhanced labeling of motor nuclei at all levels of the brain stem and spinal cord (oculomotor, trochlear, abducens, facial, trigeminal, vagal, hypoglossal, cervical, and lumbar). There was also extensive labeling of trigeminal and spinal primary afferent fibers, bulk labeling of the area postrema, and finally numerous labeled neurons in the periventricular and supraoptic hypothalamic nuclei. Generalized labeling of motor, sensory, and hypothalamic neurons could also be produced on a more limited scale from intramuscular injections of 500 microg of CTb in the tongue. Neuronal uptake of peripherally administered CTb may be useful as a research tool, or, when fused to therapeutic peptides, enzymes, growth factors, or gene therapy vectors, may have application in amyotrophic lateral sclerosis, diabetic neuropathy, motor neuronopathic lysosomal storage diseases, and other neurodegenerative disorders. |
| Databáze: | OpenAIRE |
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