Tetrahydroxystilbene glucoside alleviates angiotensin II induced HUVEC senescence via SIRT1
| Autor: | Yan Guo, Shuyu Cao, Yuefeng Xie, Shi-dong Cao, Wenxue Fan, Bo Jin, Xiangdong Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Senescence Active ingredient Polygonum biology Physiology Chemistry Angiotensin II General Medicine Tetrahydroxystilbene glucoside biology.organism_classification Glucosides Sirtuin 1 Physiology (medical) Stilbenes Human Umbilical Vein Endothelial Cells Humans Human umbilical vein endothelial cell Cellular Senescence Signal Transduction |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 99:389-394 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2020-0202 |
| Popis: | Tetrahydroxystilbene glucoside (TSG), an active ingredient of Polygonum multiflorum, has been known for certain anti-aging effects. In this study, the possible protective mechanism of TSG on human umbilical vein endothelial cell (HUVEC) senescence induced by angiotensin II (Ang II) was investigated. The results revealed that TSG pretreatment could reduce the percentage of senescence-associated β-galactosidase (SA-β-gal) positive cells and decrease the expression levels of the cellular senescence biomarker proteins p53 and PAI-1. At the same time, the expression of SIRT1 in senescent cells showed an upward trend due to TSG treatment. When inhibiting the expression of SIRT1 by EX527, our results showed that TSG reversed the effect of EX527 by promoting the expression level of SIRT1, reducing the expression of SA-β-gal positive cells and the expression level of p53 and PAI-1 proteins. The present study demonstrated that TSG could protect against HUVEC senescence induced by Ang II, potentially through modulation of SIRT1 activity. |
| Databáze: | OpenAIRE |
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