Fluorescent Treponemal Antibody Absorption Double-Staining Test Evaluation
| Autor: | Carol E. Farshy, Edward J. Kennedy, E F Hunter, Sandra A. Larsen |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Test evaluation Immunology Fluorescent Antibody Technique urologic and male genital diseases Syphilis Serodiagnosis medicine Humans Reference standards Microscopy Staining and Labeling business.industry Reference Standards Fluorescent Treponemal Antibody Absorption bacterial infections and mycoses medicine.disease female genital diseases and pregnancy complications Test (assessment) Double staining Evaluation Studies as Topic Syphilis business Nuclear medicine Reporting system |
| Zdroj: | Journal of Clinical Microbiology. 17:245-248 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.17.2.245-248.1983 |
| Popis: | The fluorescent treponemal antibody absorption (FTA-ABS) double-staining (DS) test has been developed for microscopes equipped with incident illumination, and the procedure offers many advantages over the FTA-ABS test when tests are performed with this equipment. In this study, 346 fresh sera, including 35 from patients with syphilis, were evaluated by the FTA-ABS DS test. Parameters for investigation included two readers, each using a different microscope; a new FTA-ABS DS test reporting system; sera heated at 56°C for 30 min versus unheated sera; and sera retested after at least 2 weeks of freezer storage. Agreement for FTA-ABS DS test readings between the two microscopes was 99%. Between-test agreement for the FTA-ABS test with the conventional reporting system and the FTA-ABS DS test with the new reporting system was 95%. Sensitivity calculations based on reactivity for the 35 syphilis sera were 94% for the FTA-ABS DS test and 91% for the FTA-ABS test. Specificity calculations based on non-reactivity of nonsyphilis sera were 98% for the FTA-ABS DS test and 93% for the FTA-ABS test. Differences in percentages appeared to be related to borderline readings in the FTA-ABS test. For example, if the same reporting system was used for the reference FTA-ABS test, the specificity was 97%. When sera were examined within 48 h, no difference was observed in results obtained with heated and unheated sera. Sera frozen for 2 weeks showed comparable results in the FTA-ABS DS test and the FTA-ABS test. These findings strongly support the recommendation that the FTA-ABS DS test be accepted as a confirmatory test for syphilis. The new reporting system for the FTA-ABS DS test would be advantageous for the reference FTA-ABS procedure. |
| Databáze: | OpenAIRE |
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