Effects of Pentoxifylline on the Adherence of Polymorphonuclear Neutrophils to Oxidant-Stimulated Human Endothelial Cells
| Autor: | Hassan Sellak, Jacques Hakim, Catherine Pasquier, Elisabeth Franzini, C. Babin-Chevaye |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
IBMX
Neutrophils Phosphodiesterase Inhibitors Pharmacology Biology Umbilical vein Pentoxifylline chemistry.chemical_compound 1-Methyl-3-isobutylxanthine Cell Adhesion Cyclic AMP medicine Humans Xanthine oxidase Cells Cultured chemistry.chemical_classification Reactive oxygen species Superoxide Isoproterenol Adrenergic beta-Agonists Oxidants Endothelial stem cell chemistry Biochemistry Endothelium Vascular Reactive Oxygen Species Cardiology and Cardiovascular Medicine Intracellular medicine.drug |
| Zdroj: | Journal of Cardiovascular Pharmacology. 25:S92-S95 |
| ISSN: | 0160-2446 |
| Popis: | Cultured human umbilical vein endothelial cells (HUVECs) treated with reactive oxygen species (ROS) show increased adherence of polymorphonuclear leukocytes (PMNs). Because pentoxifylline (PTX) is known to inhibit cell interactions, we studied PMN adherence to ROS-stimulated HUVECs pretreated with PTX. ROS were generated by the oxidation of hypoxanthine by xanthine oxidase, giving rise to superoxide anion and hydrogen peroxide. Human PMNs were then added to HUVEC monolayers. After various times, the cultures were washed and the number of adherent PMNs was estimated by measuring myeloperoxidase in the total cell homogenate. PTX inhibited adherence in a concentration-dependent manner. Moreover, the increase in intracellular cAMP content varied with the PTX concentration. Isobutylmethylxanthine (IBMX) and isoproterenol (ISO), which increase intracellular cAMP content, also inhibited the adherence of PMNs to ROS-stimulated HUVECs. We conclude that cAMP is probably involved in the intracellular regulation of ROS-mediated PMN adherence to endothelial cells. |
| Databáze: | OpenAIRE |
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