Gastroprotective effect and mechanism of patchouli alcohol against ethanol, indomethacin and stress-induced ulcer in rats
Autor: | Yu-Hong Liu, Xiao-Dan Yu, Ping Huang, Yi-Feng Zheng, Zi-Ren Su, Xiao-Ying Chen, Yong-Zhuo Liang, Weiwen Jiang, Jian-Hui Xie, Yifei Xu, Zhi-Zhun Mo |
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Rok vydání: | 2014 |
Předmět: |
Male
Antioxidant medicine.medical_treatment Indomethacin Stimulation Pharmacology Toxicology Dinoprostone Rats Sprague-Dawley chemistry.chemical_compound Pepsin Stress Physiological Oral administration Malondialdehyde medicine Animals RNA Messenger Stomach Ulcer Sulfhydryl Compounds Prostaglandin E2 Ethanol biology Chemistry General Medicine Glutathione Anti-Ulcer Agents Catalase digestive system diseases Rats Mucus Biochemistry Gastric Mucosa Prostaglandin-Endoperoxide Synthases biology.protein Cytokines Cyclooxygenase Sesquiterpenes Drugs Chinese Herbal medicine.drug Prostaglandin E |
Zdroj: | Chemico-Biological Interactions. 222:27-36 |
ISSN: | 0009-2797 |
Popis: | Pogostemonis Herba is an important Chinese medicine widely used in the treatment of gastrointestinal dysfunction. Patchouli alcohol (PA), a tricyclic sesquiterpene, is the major active constituent of Pogostemonis Herba. This study aimed to investigate the possible anti-ulcerogenic potential of PA and the underlying mechanism against ethanol, indomethacin and water immersion restraint-induced gastric ulcers in rats. Gross and histological gastric lesions, biochemical and immunological parameters were taken into consideration. The gastric mucus content and the antisecretory activity were analyzed through pylorus ligature model in rats. Results indicated that oral administration with PA significantly reduced the ulcer areas induced by ethanol, indomethacin and water immersion restraint. PA pretreatment significantly promoted gastric prostaglandin E2 (PGE2) and non-protein sulfhydryl group (NP-SH) levels, upregulated the cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) mRNA expression, and considerably boosted the gastric blood flow (GBF) and gastric mucus production in comparison with vehicle. In addition, PA modulated the levels of interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α). The levels of glutathione (GSH), catalase (CAT) and malonaldehyde (MDA) were also restored by PA. However, the gastric secretion parameters (pH, volume of gastric juice and pepsin) did not show any significant alteration. These findings suggest that PA exhibited significant gastroprotective effects against gastric ulceration. The underlying mechanisms might involve the stimulation of COX-mediated PGE2, improvement of antioxidant and anti-inflammatory status, preservation of GBF and NP-SH, as well as boost of gastric mucus production. |
Databáze: | OpenAIRE |
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