Detecting methylation patterns ofp16,MGMT,DAPKandE-cadheringenes in multiple myeloma patients
| Autor: | E. Kizilkilic, N. E. Kocer, O. Ozalp Yuregir, Feride Iffet Sahin, Erkan Yurtcu, Hakan Ozdogu |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Clinical Biochemistry Biology Polymerase Chain Reaction law.invention Pathogenesis law Biomarkers Tumor Humans Epigenetics Promoter Regions Genetic DNA Modification Methylases Gene Polymerase chain reaction Aged Clinical Laboratory Techniques Cadherin Tumor Suppressor Proteins Biochemistry (medical) Promoter Hematology General Medicine Methylation DNA Methylation Middle Aged Cadherins Molecular biology Death-Associated Protein Kinases DNA Repair Enzymes Calcium-Calmodulin-Dependent Protein Kinases DNA methylation Cancer research Female Apoptosis Regulatory Proteins Multiple Myeloma |
| Zdroj: | International Journal of Laboratory Hematology. 32:142-149 |
| ISSN: | 1751-553X 1751-5521 |
| DOI: | 10.1111/j.1751-553x.2009.01146.x |
| Popis: | Summary Multiple myeloma (MM) is a B-cell neoplasia characterized by the clonal proliferation of plasma cells. Besides known genetic abnormalities, epigenetic changes are also known to effect MM pathogenesis. DNA methylation is an epigenetic mechanism that silences genes by adding methyl groups to cytosine-guanine dinucleotides at the promoter regions. In this study, the methylation status of four genes; p16, O6-methyl guanine DNA methyl transferase (MGMT), death-associated protein kinase (DAPK) and E-cadherin (ECAD); at the time of diagnosis was investigated using methylation-specific polymerase chain reaction (MS-PCR). In the 20 cases studied; methylation of the promoter regions of p16, MGMT, DAPK and ECAD genes was detected in 10%, 40%, 10% and 45% of the cases, respectively. In 65% (13/20) of cases, at least one of the genes studied had promoter methylation; while 35% of cases (7/20) had methylated promoters of more than one gene. There was a significant correlation between promoter hypermethylation of MGMT and the presence of extramedullary involvement; but for the other genes no correlation was found regarding disease properties like age, disease stage, clinical course and the presence of lytic bone lesions. Determining the methylation profiles of genes in MM, could lead to a new understanding of the disease pathogenesis and guide the assessment of treatment options. |
| Databáze: | OpenAIRE |
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