Co-emergence of cardiac and gut tissues promotes cardiomyocyte maturation within human iPSC-derived organoids
| Autor: | Michael H. Lai, Andrew P. Blair, Oriane B. Matthys, David A Joy, Benoit G. Bruneau, Michael Alexanian, Ana C. Silva, Mara A. Kauss, Todd C. McDevitt, Vaishaali Natarajan, Diwaker Turaga |
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| Rok vydání: | 2020 |
| Předmět: |
Pluripotent Stem Cells
Embryogenesis Endoderm Induced Pluripotent Stem Cells Morphogenesis Cell Differentiation Cell Biology Cell fate determination Biology Cell biology Organoids Paracrine signalling medicine.anatomical_structure Genetics medicine Organoid Molecular Medicine Humans Myocytes Cardiac NODAL Induced pluripotent stem cell |
| Zdroj: | Cell stem cell. 28(12) |
| ISSN: | 1875-9777 |
| Popis: | Summary During embryogenesis, paracrine signaling between tissues in close proximity contributes to the determination of their respective cell fate(s) and development into functional organs. Organoids are in vitro models that mimic organ formation and cellular heterogeneity, but lack the paracrine input of surrounding tissues. Here, we describe a human multilineage iPSC-derived organoid that recapitulates cooperative cardiac and gut development and maturation, with extensive cellular and structural complexity in both tissues. We demonstrate that the presence of endoderm tissue (gut/intestine) in the organoids contributed to the development of cardiac tissue features characteristic of stages after heart tube formation, including cardiomyocyte expansion, compartmentalization, enrichment of atrial/nodal cells, myocardial compaction, and fetal-like functional maturation. Overall, this study demonstrates the ability to generate and mature cooperative tissues originating from different germ lineages within a single organoid model, an advance that will further the examination of multi-tissue interactions during development, physiological maturation, and disease. |
| Databáze: | OpenAIRE |
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