Coupling High-Performance Thin-Layer Chromatography with Bacterial Genotoxicity Bioreporters
| Autor: | Georg Reifferscheid, Liat Moscovici, Dror Shakibai, Sebastian Buchinger, Carolin Riegraf, Shimshon Belkin |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
biology Chemistry General Chemistry 010501 environmental sciences medicine.disease_cause biology.organism_classification 01 natural sciences Gene cassette Photorhabdus luminescens Escherichia coli medicine Environmental Chemistry Bioluminescence Chromatography Thin Layer Bioreporter High performance thin layer chromatography Photorhabdus Effluent Genotoxicity DNA Damage Plasmids 0105 earth and related environmental sciences |
| Zdroj: | Environmental Science & Technology. 53:6410-6419 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.9b00921 |
| Popis: | We present an innovative technological platform for monitoring the direct genotoxicity of individual components in complex environmental samples, based on bioluminescent Escherichia coli genotoxicity bioreporters, sprayed onto the surface of a high-performance thin-layer chromatography (HPTLC) plate. These sensor strains harbor plasmid-borne fusions of selected gene promoters of the E. coli SOS DNA repair system to the Photorhabdus luminescens luxABCDE gene cassette, and mark by increased luminescence the presence of potentially DNA-damaging sample components separated on the plate. We demonstrate an "on plate" quantifiable dose-dependent response to several model genotoxicants (without metabolic activation). We further demonstrate the applicability of the system by identifying as genotoxic specific components of HPTLC-separated influent and effluent samples of wastewater treatment plants, thereby alleviating the need for a comprehensive chemical analysis of the sample. |
| Databáze: | OpenAIRE |
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