A novel assay for erythrocyte carbonic anhydrase and certain sulfonamide drugs employing spin-labels
| Autor: | Donnas Starkweather, Colin F. Chignell |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Erythrocytes
Chemical Phenomena Dichlorphenamide In Vitro Techniques chemistry.chemical_compound Carbonic anhydrase medicine Methods Animals Humans Spin label Carbonic Anhydrases Pharmacology chemistry.chemical_classification Sulfonamides biology Aryl Electron Spin Resonance Spectroscopy Active site General Medicine Sulfonamide Acetazolamide Chemistry Enzyme chemistry Biochemistry Spectrophotometry biology.protein Cattle Hemoglobin medicine.drug |
| Zdroj: | Pharmacology. 8(4) |
| ISSN: | 0031-7012 |
| Popis: | A novel assay procedure has been developed for the determination of sulfonamide drugs with the general formula ArSO2NH2 where Ar = aryl (either homocyclic or heterocyclic). The assay depends on the displacement of a spin-labeled sulfonamide from the active site of bovine carbonic anhydrase B and can detect concentrations of sulfonamide as low as 0.3 µg/ml. This technique can also be used to assay low concentrations of carbonic anhydrase in hemolysates of red blood cells without prior removal of the hemoglobin or isolation of the enzyme. |
| Databáze: | OpenAIRE |
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