Functional correlation between kallikrein and factor XII activated in human plasma
| Autor: | Nils-Ove Hoem, Kjell Briseid, Siri Johannesen, Kari Marthinsen |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Male High-molecular-weight kininogen Immunoblotting Factor XIIa urologic and male genital diseases Benzamidine chemistry.chemical_compound Aprotinin Amidase activity Humans Factor XII Kininogen Kunitz STI protease inhibitor Kininogens urogenital system Chemistry Prekallikrein Hematology Kallikrein Middle Aged Benzamidines Biochemistry Chromatography Gel Kallikreins Oligopeptides circulatory and respiratory physiology |
| Zdroj: | Thrombosis Research. 57:945-956 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/0049-3848(90)90161-5 |
| Popis: | Plasma kallikrein and FXIIa were assayed in acetone-treated human citrated plasma (CPLa) with the chromogenic peptide Bz-Ile-Glu-Gly-Arg-pNA (S-2222) as substrate. In end point assays with short incubation periods (1–10 min.) nearly all kallikrein present could be blocked by a low concentration of soybean trypsin inhibitor (STI). In 30 min. assays the main part of the kallikrein was recovered in a functional state not inhibited by STI, and at the same time the level of FXIIa (as amidase activity blocked by corn inhibitor, C.I.) was reduced to about 2/3 of the initial value. The formation of an association between FXIIa and kallikrein is suggested. In fractions from gel filtration of CPLa kallikrein was assayed as S-2302 amidase, high molecular weight kininogen (HK) was measured in rocket immunoassays, and HK and FXII were studied in PAGE immunoblot experiments. Kallikrein appeared as one peak together with HK (gel mol. wt. 300 KD), about 40% of HK was free (220 KD), and no FXII was observed in the kallikrein or HK peaks, but in two areas corresponding to 78–79 KD and 39–42 KD. When experiments, however, were carried out with plasma acetone-activated and gel filtered in the presence of benzamidine (5 mM), part of the amidase activity present in kallikrein peak fractions was blocked by C.I. This observation supports the above suggestion of an association between FXIIa and kallikrein. |
| Databáze: | OpenAIRE |
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