Oral and Topical Insecticide Response Bioassays and Associated Statistical Analyses Used Commonly in Veterinary and Medical Entomology
| Autor: | Christopher J. Geden, Edwin R Burgess, Bethia H. King |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
AcademicSubjects/SCI01382 Veterinary medicine Insecticides Medical entomology probit analysis Spinosad Biology 01 natural sciences R programming chemistry.chemical_compound Houseflies parasitic diseases Nitriles Pyrethrins medicine Bioassay Animals pesticide Pyrethroid business.industry Special Collection: Protocols in Medical and Veterinary Entomology fungi Pest control General Medicine Pesticide biology.organism_classification 010602 entomology Drug Combinations house fly chemistry Insect Science Muscidae Biological Assay Female PEST analysis Macrolides business Protocols 010606 plant biology & botany medicine.drug toxicology |
| Zdroj: | Journal of Insect Science |
| ISSN: | 1536-2442 |
| Popis: | Veterinary and medical entomologists who are involved in research on pest control often need to perform dose–response bioassays and analyze the results. This article is meant as a beginner’s guide for doing this and includes instructions for using the free program R for the analyses. The bioassays and analyses are described using previously unpublished data from bioassays on house flies, Musca domestica Linnaeus (Diptera: Muscidae), but can be used on a wide range of pest species. Flies were exposed topically to beta-cyfluthrin, a pyrethroid, or exposed to spinosad or spinetoram in sugar to encourage consumption. LD50 values for beta-cyfluthrin in a susceptible strain were similar regardless of whether mortality was assessed at 24 or 48 h, consistent with it being a relatively quick-acting insecticide. Based on LC50 values, spinetoram was about twice as toxic as spinosad in a susceptible strain, suggesting a benefit to formulating spinetoram for house fly control, although spinetoram was no more toxic than spinosad for a pyrethroid-resistant strain. Results were consistent with previous reports of spinosad exhibiting little cross-resistance. For both spinosad and spinetoram, LC50 values were not greatly different between the pyrethroid-resistant strain and the susceptible strain. |
| Databáze: | OpenAIRE |
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