Identification and functional analysis of long non-coding RNAs in human and mouse early embryos based on single-cell transcriptome data
| Autor: | Jia-jun Qiu, Jing-bin Yan, Zhao-rui Ren |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Zygote Embryonic Development Rett syndrome Biology Morula Epigenesis Genetic 03 medical and health sciences Mice 0302 clinical medicine early embryo medicine Human embryogenesis Animals Humans human embryogenesis Epigenetics Gene Genetics Genome Models Statistical single-cell RNA-seq Genome Human Sequence Analysis RNA WGCNA Gene Expression Profiling Cell Cycle Gene Expression Regulation Developmental Embryo medicine.disease Embryonic stem cell long non-coding RNAs (lncRNAs) 030104 developmental biology Blastocyst Oncology ROC Curve 030220 oncology & carcinogenesis Oocytes Medical genetics Maternal to zygotic transition RNA Long Noncoding Single-Cell Analysis Transcriptome Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Jia-jun Qiu 1 , Zhao-rui Ren 1, 2 , Jing-bin Yan 1, 2 1 Shanghai Children’s Hospital, Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University School of Medicine, Shanghai 200040, China 2 Key Laboratory of Embryo Molecular Biology, Ministry of Health of China and Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200040, China Correspondence to: Zhao-rui Ren, email: zhrren1225@163.com Jing-bin Yan, email: yanjingbin0130@hotmail.com Keywords: long non-coding RNAs (lncRNAs), early embryo, WGCNA, single-cell RNA-seq, human embryogenesis Received: January 21, 2016 Accepted: August 08, 2016 Published: August 16, 2016 ABSTRACT Epigenetics regulations have an important role in fertilization and proper embryonic development, and several human diseases are associated with epigenetic modification disorders, such as Rett syndrome, Beckwith-Wiedemann syndrome and Angelman syndrome. However, the dynamics and functions of long non-coding RNAs (lncRNAs), one type of epigenetic regulators, in human pre-implantation development have not yet been demonstrated. In this study, a comprehensive analysis of human and mouse early-stage embryonic lncRNAs was performed based on public single-cell RNA sequencing data. Expression profile analysis revealed that lncRNAs are expressed in a developmental stage–specific manner during human early-stage embryonic development, whereas a more temporal-specific expression pattern was identified in mouse embryos. Weighted gene co-expression network analysis suggested that lncRNAs involved in human early-stage embryonic development are associated with several important functions and processes, such as oocyte maturation, zygotic genome activation and mitochondrial functions. We also found that the network of lncRNAs involved in zygotic genome activation was highly preservative between human and mouse embryos, whereas in other stages no strong correlation between human and mouse embryo was observed. This study provides insight into the molecular mechanism underlying lncRNA involvement in human pre-implantation embryonic development. |
| Databáze: | OpenAIRE |
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