Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China
| Autor: | Longxian Zhang, Meng Qi, Yixuan Zhu, Zhao Aiyun, Ying Zhang, Yushi Peng, Zilin Wei, Dongfang Li |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Enterocytozoon bieneusi genotype prevalence lcsh:QR1-502 racehorse Microbiology lcsh:Microbiology 03 medical and health sciences fluids and secretions parasitic diseases Genotype Parasite hosting Internal transcribed spacer zoonotic Genotyping Feces 030304 developmental biology 0303 health sciences biology Phylogenetic tree 030306 microbiology fungi virus diseases biology.organism_classification Virology Nested polymerase chain reaction |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2019.01920 |
| Popis: | Enterocytozoon bieneusi is a widely distributed human and animal pathogen. However, few data are available on the distribution of E. bieneusi genotypes in racehorses. In this study, 621 fecal specimens were collected from racehorses at 17 equestrian clubs in 15 Chinese cities. E. bieneusi was detected via nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) gene. The overall infection rate of E. bieneusi was 4.8% (30/621). Statistically significant differences were found in the prevalence of this parasite among the equestrian clubs (χ2 = 78.464, df = 16, p < 0.01) and age groups (χ2 = 23.686, df = 1, p < 0.01), but no sex bias was found among the racehorses for the E. bieneusi infections (χ2 = 1.407, df = 2, p > 0.05). Ten E. bieneusi genotypes were identified, including seven known genotypes (EbpC, EbpA, Peru6, horse1, horse2, CAF1, and TypeIV) and three novel genotypes (HBH-1, SXH-1, and BJH-1). Phylogenetic analysis showed that EbpC, EbpA, Peru6, horse2, CAF1, TypeIV, BJH-1, and SXH-1 belonged to Group 1 of E. bieneusi, HBH-1 belonged to Group 2, and horse2 belonged to Group 6. Our findings advance the current knowledge of E. bieneusi prevalence and genotypes in racehorses in China. |
| Databáze: | OpenAIRE |
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