Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connections

Autor: Arthur Liesz, Shan Zhao, Martin Kerschensteiner, Chenchen Pan, Hanno Steinke, Arnaldo Parra-Damas, Ruiyao Cai, Corinne Benakis, Markus Rempfler, Ali Ertürk, Mihail Ivilinov Todorov, Harsharan S. Bhatia, Sabine Liebscher, Anna L.R. Xavier, Bjoern H. Menze, Benjamin T. Kress, Benjamin Förstera, Delphine Theodorou, Ingo Bechmann, Leander Mrowka, Alireza Ghasemigharagoz
Rok vydání: 2018
Předmět:
Zdroj: Nature neuroscience
ISSN: 1546-1726
1097-6256
DOI: 10.1038/s41593-018-0301-3
Popis: Analysis of entire transparent rodent bodies after clearing could provide holistic biological information in health and disease, but reliable imaging and quantification of fluorescent protein signals deep inside the tissues has remained a challenge. Here, we developed vDISCO, a pressure-driven, nanobody-based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image and quantify subcellular details through bones, skin and highly autofluorescent tissues of intact transparent mice. For the first time, we visualized whole-body neuronal projections in adult mice. We assessed CNS trauma effects in the whole body and found degeneration of peripheral nerve terminals in the torso. Furthermore, vDISCO revealed short vascular connections between skull marrow and brain meninges, which were filled with immune cells upon stroke. Thus, our new approach enables unbiased comprehensive studies of the interactions between the nervous system and the rest of the body.
Databáze: OpenAIRE
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