The Search for Molecular Prognostic Markers of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus
| Autor: | M. M. Batiushin, V. Ibragimov, Irina V. Sarvilina |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Pathology Functional features type 2 diabetes mellitus 030232 urology & nephrology proteomics lcsh:Medicine Urine 030204 cardiovascular system & hematology Gastroenterology molecular markers General Biochemistry Genetics and Molecular Biology Diabetic nephropathy 03 medical and health sciences 0302 clinical medicine Internal medicine Medicine In patient Molecular interactions General Immunology and Microbiology business.industry General Neuroscience lcsh:R diabetic nephropathy Type 2 Diabetes Mellitus medicine.disease Overt nephropathy Healthy individuals business |
| Zdroj: | International Journal of Biomedicine, Vol 6, Iss 1, Pp 65-69 (2016) |
| ISSN: | 2158-0529 2158-0510 |
| DOI: | 10.21103/article6(1)_oa14 |
| Popis: | The purpose of this study was to search for molecular prognostic markers of diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (T2DM). The study included 205 patients with T2DM and DN (stages 1 to 4). All patients were stratified by the MDRD equation. The control group included 30 healthy individuals. All T2DM patients were divided into 4 groups depending on the DN stages. Group 1 included 42 patients with DN-Stage 1 (prenephropathy), Group 2 included 48 patients with DN-Stage 2 (incipient nephropathy); Group 3 included 65 patients with DN-Stage 3 (overt nephropathy), and Group 4 included 50 patients with DN-Stage 4 (kidney failure). Molecular phenotyping of urine was processed with methods of proteomics: the prefractionation, the separation of proteins with standard sets (MB-HIC C8 Kit, MB-IMAC Cu, MB-Wax Kit, «Bruker», USA), matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS, Ultraflex II, «Bruker», USA). The data of the molecular interactions and functional features of proteins were received with STRING 10.0 database. Potentially new molecular markers of DN development were identified. The research into signaling pathways and the molecules that are involved in ECM formation may help in developing strategies to prevent DN. |
| Databáze: | OpenAIRE |
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