Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF and CD34 in diabetic ulcer mouse model of wound healing
| Autor: | M. Restiwijaya, Prasetyowati Subchan, Wibi Riawan, Renni Yuniati, Niken Safitri Dyan Kusumaningrum, Muhammad Nur, Matthew Brian Khrisna |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
food.ingredient viruses VEGF receptors CD34 Diabetic ulcers General Biochemistry Genetics and Molecular Biology Diabetic wound 03 medical and health sciences 0302 clinical medicine food Diabetes mellitus medicine General Pharmacology Toxicology and Pharmaceutics integumentary system General Immunology and Microbiology biology business.industry Coconut oil virus diseases General Medicine biochemical phenomena metabolism and nutrition medicine.disease digestive system diseases 030104 developmental biology 030220 oncology & carcinogenesis Anesthesia biology.protein business Wound healing Complication |
| Zdroj: | F1000Research. 9:580 |
| ISSN: | 2046-1402 |
| Popis: | Background: Diabetes is a disease that affects people worldwide, including in Indonesia. The prevalence of diabetes in Indonesia is increasing from year to year. One of the most devastating complications of diabetes mellitus is diabetic ulcers, which is a limb-threatening complication. Over the past few decades, ozone generated using plasma medical technology has been investigated as an agent that helps wound healing. This study aims to evaluate the effects of topical ozonated virgin coconut oil (VCO) in a diabetic wound mouse model. Methods: This study was an experimental study with a post-test control design. An ulcer wound model was made in 50 diabetic male Wistar mice, divided into five groups, and a control group of 10 non-diabetic mice. The control groups were given conventional therapy only and the treatment groups were also given topical ozonated VCO with different flow durations (0 min, 90 min, 7 h, 14 h). Macroscopic appearance and wound contraction were observed. HSP90β, VEGF-A, EGF, bFGF, and CD34 levels were measured from the immunostained slices of wound margins. Results: The reduction of wound length was proportionally related to the duration of ozone flow. Ozonated VCO with a longer duration of ozone flow healed the wound more quickly and had the shortest wound length. VCO with ozone flow for 14 hours (16837.10 µm) had the biggest reduction in wound length compared to other groups. The wounds treated with ozonated VCO showed an increase in HSP90β, VEGF-A, EGF, bFGF, and CD34 levels that correlated to improved wound healing. A longer period of treatment resulted in higher levels of wound healing biomarkers compared to shorter therapeutic durations. Conclusions: Topical ozonated VCO improved the wound healing process in a diabetic ulcer mouse model by improving macroscopic wound appearance and increasing levels of wound healing biomarkers. |
| Databáze: | OpenAIRE |
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