Evidence for a TSH-Controlled Ectophosphotyrosine Phosphatase in Pig Thyroid Cultured Cells
| Autor: | Gustave Aguie-Aguie, Hervé Sartelet, Bernard Haye, Emmanuelle Petitfrère, Laurent Martiny |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Swine Phosphatase Tetramisole Thyroid Gland Biophysics Morphogenesis Thyrotropin Cell Communication Protein tyrosine phosphatase Biochemistry Iodine Radioisotopes Chlorides Thyroid peroxidase Internal medicine medicine Extracellular Animals Viability assay Enzyme Inhibitors Phosphotyrosine Molecular Biology Cells Cultured Molybdenum biology Cell Biology Hydrogen-Ion Concentration Iodides Phosphoproteins Trypsin Kinetics Endocrinology Zinc Compounds biology.protein Sodium Fluoride Phosphorylation Protein Tyrosine Phosphatases Vanadates hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 220:746-753 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.0475 |
| Popis: | In porcine thyroid cells in primary culture, thyrotropin (TSH) provokes important morphological changes associated with specific thyroidal function recovery. TSH influences cell–cell interactions and follicular morphogenesis. In the present report, we identify an ectotyrosine phosphatase activity controlled by chronic treatment with TSH. Specific substrates and various inhibitors allowed us to characterize this activity. This tyrosine phosphatase is located at the outer surface of thyroid cells as demonstrated by phosphotyrosylhistone hydrolysis. Control of cell viability and trypsin treatment confirm this extracellular localization. These data show that TSH activates ectotyrosine phosphatase activity, suggesting a role for surface protein phosphorylation in regulating specific functions of porcine thyroid cells in suspension. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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