Streptozotocin-induced diabetes is associated with altered expression of peptide-encoding mRNAs in rat sensory neurons
| Autor: | Richard M. Kream, Susan E. Leeman, Peter A. Rittenhouse, James E. Marchand, Juan Chen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Preprotachykinin Physiology Calcitonin Gene-Related Peptide Radioimmunoassay Gene Expression Neuropeptide Substance P In situ hybridization Calcitonin gene-related peptide Biochemistry Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Dorsal root ganglion Ganglia Spinal Internal medicine Animals Medicine Neuropeptide Y Neurons Afferent RNA Messenger Protein Precursors In Situ Hybridization business.industry Neuropeptides Streptozotocin Neuropeptide Y receptor Rats medicine.anatomical_structure chemistry Somatostatin business medicine.drug |
| Zdroj: | Peptides. 17:1017-1022 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/0196-9781(96)00129-5 |
| Popis: | Major complications arising from diabetes mellitus include neuropathic pain and altered peripheral inflammatory responses. Somatostatin (SOM), calcitenin gene-related peptide (CGRP), and substance P (SP) are neuropeptides that modulate pain responses transmitted by primary sensory afferents, the cell bodies of which are located in the dorsal root ganglion (DRG). Thus, the goal of the present study was to determine whether the diabetic condition is associated with altered neuropeptide gene expression in lumbar DRG of the rat. We employed an established animal model in which streptozotocin (STZ, 55 mg/kg) is administered to 6 week-old rats. The hallmark symptoms of hyperglycemia (blood glucose400 mg/dl), polydipsia, polyuria, and severe weight loss were maximal at 6 weeks postadministration, at which time animals were sacrificed. For determination of peptide encoding mRNAs distributed in DRG neurons, in situ hybridization histochemistry utilizing S-end-labeled oligonucleotides complimentary to sequences of preprosomatostatin (PPSOM), preprocalcitonin gene related peptide (PPCGRP), preprotachykinin (PPT), or preproneuropeptide Y (PPNPY) mRNA was performed. Silver grains were detected overlying DRG cells by autoradiography on sections of tissue counterstained with thionin. Semiquantitative analysis of differences in silver grain signal were made using an image analysis system, which expressed signals as fCi/microns2. In diabetic rats there was a significant decrease in DRG PPSOM (54%, p0.01), and PPCGRP (33%. p0.05) mRNA hybridization from the normal values PPT mRNA hybridization signal and SP-like immunoreactivity were not significantly changed in diabetic rat DRGs compared to control. In contrast, there was an increase in the number of cells labeled with PPNPY hybridization in DRG from diabetic rats. These data suggest that CGRP and SOM synthesis in primary sensory neurons is reduced in STZ-induced diabetic rats. These changes could contribute to the painful neuropathies and altered inflammatory responses seen in diabetes mellitus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect