Neuroblasts Micropatterning On Nanostructural Modified Chitosan Membranes

Autor: Chun-Yen Sung, Chung-Yao Yang, Tzu-Chun Liao, Wen-Shiang Chen, Chao-Min Cheng, J. Andrew Yeh
Jazyk: angličtina
Rok vydání: 2013
Předmět:
DOI: 10.5281/zenodo.1058198
Popis: The study describes chitosan membrane platform modified with nanostructure pattern which using nanotechnology to fabricate. The cell-substrate interaction between neuro-2a neuroblasts cell lines and chitosan membrane (flat, nanostructure and nanostructure pattern types) was investigated. The adhered morphology of neuro-2a cells depends on the topography of chitosan surface. We have found that neuro-2a showed different morphogenesis when cells adhered on flat and nanostructure chitosan membrane. The cell projected area of neuro-2a on flat chitosan membrane is larger than on nanostructure chitosan membrane. In addition, neuro-2a cells preferred to adhere on flat chitosan surface region than on nanostructure chitosan membrane to immobilize and differentiation. The experiment suggests surface topography can be used as a critical mechanism to isolate group of neuro-2a to a particular rectangle area on chitosan membrane. Our finding will provide a platform to take patch clamp to record electrophysiological behavior about neurons in vitro in the future.
{"references":["N. Li and A. Folch, \"Integration of topographical and biochemical cues\nby axons during growth on microfabricated 3-D substrates,\" Exp. Cell\nRes., vol. 311, pp. 307- 316, Nov. 2005.","H. G. Craighead, S. W. Turner, R. C. Davis, C. James, A. M. Perez, P. M.\nSt. John, M. S. Isaacson, L. Kam, W. Shain, J. N. Turner and G. Banker,\n\"Chemical and Topographical Surface Modification for Control of\nCentral Nervous System Cell Adhesion,\" Biomed. Microdevices, vol. 1,\npp. 49-64, Sep. 1998.","M. Arnold, E. A. C. Adam, R. Glass, J. B. Mmel, W. Eck, M. Kantlehner,\nH. Kessler, and J. -P. Spatz, \"Induction of Cell Polarization and Migration\nby a Gradient of Nanoscale Variations in Adhesive Ligand Spacing,\"\nNano Lett., vol. 8, pp. 2063-2069, June 2008.","B. Zhua, Q. Zhangb, Q. Lua, Y. Xub, J. Yina, J. Hub and Z. Wanga,\n\"Nanotopographical guidance of C6 glioma cell alignment and oriented\ngrowth,\" Biomaterials, vol. 25, pp. 4215-422, Aug. 2004.","M. Cecchini, G. Bumma, M. Serresi and F. Beltram, \"PC12\ndifferentiation on biopolymer nanostructures,\" Nanotechnology, vol.18,\npp.505103, Nov. 2007.","M. Cheng, J. Deng, F. Yang, Y. Gong, N. Zhao and X. Zhang, \"Study on\nphysical properties and nerve cell affinity of composite films from\nchitosan and gelatin solutions,\" Biomaterials, vol. 24, pp. 2871-2880,\nAug. 2003.","G. Haipeng, Z. Yinghui, L. Jianchun, G. Yandao, Z. Nanming and Z.\nXiufang, \"Studies on nerve cell affinity of chitosan-derived materials,\" J.\nBiomed. Mater. Res., vol. 52, pp. 285-95, Nov. 2000."]}
Databáze: OpenAIRE
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