Standardized susceptibility testing of fluconazole: an international collaborative study

Autor: B Dupont, P F Troke, M A Pfaller, A Espinel-Ingroff, T J Walsh, D W Warnock, J Müller, S Shadomy, M G Rinaldi, G S Kobayashi
Rok vydání: 1992
Předmět:
Zdroj: Antimicrobial Agents and Chemotherapy. 36:1805-1809
ISSN: 1098-6596
0066-4804
DOI: 10.1128/aac.36.9.1805
Popis: An international collaborative study of broth dilution (MIC) and disk diffusion susceptibility testing of fluconazole was conducted by using a chemically defined medium (High-Resolution Antifungal Assay Medium; Oxoid Ltd., Basingstoke, United Kingdom) and standard test methods performed in eight reference laboratories. Ten yeast isolates were tested by each test method in duplicate on each of 3 separate days. The intralaboratory reproducibility of the MIC test was excellent; 95.7% of the replicate tests (n = 220) were within 2 doubling dilutions of the other values in the set for the eight laboratories. The intralaboratory reproducibility of the disk test was also good, with 91% of the replicate tests (n = 234) agreeing with each other within an arbitrarily chosen value of 4 mm. Interlaboratory agreement of MIC test results was acceptable, with 84% of the MICs agreeing within 2 doubling dilutions. In contrast, the interlaboratory agreement of the disk test was not good, with only 59% of test results agreeing within 4 mm. Comparison of the rank order of MICs obtained in each laboratory with the reference rank order gave an agreement of 70 to 80% (median, 80%) with the MIC test and 70 to 90% (median, 80%) with the disk test. These preliminary results are encouraging for the development of standardized testing methods for testing fluconazole.
Databáze: OpenAIRE
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