Neuronal contact guidance and YAP signaling on ultra-small nanogratings
| Autor: | Ilaria Tonazzini, Marco Cecchini, Eleonora Savi, Agnese Sonato, Cecilia Masciullo, Filippo Romanato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Neurite Confocal lcsh:Medicine 02 engineering and technology Hippocampus PC12 Cells Article Biomaterials Focal adhesion 03 medical and health sciences chemistry.chemical_compound Cell Adhesion Neurites Animals lcsh:Science Cell adhesion Multidisciplinary Total internal reflection fluorescence microscope lcsh:R YAP-Signaling Proteins 021001 nanoscience & nanotechnology Rats Axon Guidance Nanostructures Nocodazole 030104 developmental biology Nerve growth factor chemistry Apoptosis Regulatory Proteins Signal Transduction Biophysics lcsh:Q Axon guidance Biomaterials - cells 0210 nano-technology Biotechnology |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-18 (2020) Scientific reports (Nature Publishing Group) 10 (2020): 3742. doi:10.1038/s41598-020-60745-5 info:cnr-pdr/source/autori:Tonazzini I.; Masciullo C.; Savi E.; Sonato A.; Romanato F.; Cecchini M./titolo:Neuronal contact guidance and YAP signaling on ultra-small nanogratings/doi:10.1038%2Fs41598-020-60745-5/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:3742/pagina_a:/intervallo_pagine:3742/volume:10 Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Contact interaction of neuronal cells with extracellular nanometric features can be exploited to investigate and modulate cellular responses. By exploiting nanogratings (NGs) with linewidth from 500 nm down to 100 nm, we here study neurite contact guidance along ultra-small directional topographies. The impact of NG lateral dimension on the neuronal morphotype, neurite alignment, focal adhesion (FA) development and YAP activation is investigated in nerve growth factor (NGF)-differentiating PC12 cells and in primary hippocampal neurons, by confocal and live-cell total internal reflection fluorescence (TIRF) microscopy, and at molecular level. We demonstrate that loss of neurite guidance occurs in NGs with periodicity below 400 nm and correlates with a loss of FA lateral constriction and spatial organization. We found that YAP intracellular localization is modulated by the presence of NGs, but it is not sensitive to their periodicity. Nocodazole, a drug that can increase cell contractility, is finally tested for rescuing neurite alignment showing mild ameliorative effects. Our results provide new indications for a rational design of biocompatible scaffolds for enhancing nerve-regeneration processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|