Shared epitope-aryl hydrocarbon receptor crosstalk underlies the mechanism of gene-environment interaction in autoimmune arthritis
| Autor: | Joseph Holoshitz, Patrick Coit, Song Ling, Vincent van Drongelen, Sarah Veloso Nogueira, Jiaqi Fu, Edward F. Rosloniec, Amr H. Sawalha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Arthritis Mice Transgenic 03 medical and health sciences Epitopes Mice 0302 clinical medicine Osteoclast medicine Animals Humans Allele Transcription factor Alleles Multidisciplinary biology medicine.disease Aryl hydrocarbon receptor Arthritis Experimental 3. Good health Cell biology Crosstalk (biology) 030104 developmental biology medicine.anatomical_structure Receptors Aryl Hydrocarbon 13. Climate action biology.protein Th17 Cells Environmental Pollutants Gene-Environment Interaction Signal transduction 030215 immunology Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 115(18) |
| ISSN: | 1091-6490 |
| Popis: | The susceptibility to autoimmune diseases is affected by genetic and environmental factors. In rheumatoid arthritis (RA), the shared epitope (SE), a five-amino acid sequence motif encoded by RA-associated HLA-DRB1 alleles, is the single most significant genetic risk factor. The risk conferred by the SE is increased in a multiplicative way by exposure to various environmental pollutants, such as cigarette smoke. The mechanism of this synergistic interaction is unknown. It is worth noting that the SE has recently been found to act as a signal transduction ligand that facilitates differentiation of Th17 cells and osteoclasts in vitro and in vivo. Intriguingly, the aryl hydrocarbon receptor (AhR), a transcription factor that mediates the xenobiotic effects of many pollutants, including tobacco combustion products, has been found to activate similar biologic effects. Prompted by these similarities, we sought to determine whether the SE and AhR signaling pathways interact in autoimmune arthritis. Here we uncovered a nuclear factor kappa B-mediated synergistic interaction between the SE and AhR pathways that leads to markedly enhanced osteoclast differentiation and Th17 polarization in vitro. Administration of AhR pathway agonists to transgenic mice carrying human SE-coding alleles resulted in a robust increase in arthritis severity, bone destruction, overabundance of osteoclasts, and IL17-expressing cells in the inflamed joints and draining lymph nodes of arthritic mice. Thus, this study identifies a previously unrecognized mechanism of gene-environment interaction that could provide insights into the well-described but poorly understood amplification of the genetic risk for RA upon exposure to environmental pollutants. |
| Databáze: | OpenAIRE |
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