Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species
| Autor: | Daniel Karcher, Yinghong Lu, Stephanie Ruf, Ralph Bock, Shreya Agrawal, Sandra Stegemann |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene Nicotiana tabacum Xanthophylls Genes Plant 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Synthetic biology Tobacco Plastids Transgenes Plastid Genome Chloroplast Nicotiana 2. Zero hunger Genetics biology fungi Gene Transfer Techniques food and beverages biology.organism_classification Chloroplast 030104 developmental biology General Agricultural and Biological Sciences 010606 plant biology & botany Transplastomic plant |
| Zdroj: | Current Biology |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2017.08.044 |
| Popis: | Summary Transgene expression from the plastid (chloroplast) genome provides unique advantages, including high levels of foreign protein accumulation, convenient transgene stacking in operons, and increased biosafety due to exclusion of plastids from pollen transmission [1, 2]. However, applications in biotechnology and synthetic biology are severely restricted by the very small number of plant species whose plastid genomes currently can be transformed [3, 4]. Here we report a simple method for the introduction of useful plastid transgenes into non-transformable species. The transgenes tested comprised a synthetic operon encoding three components of a biosynthetic pathway for producing the high-value ketocarotenoid astaxanthin in the plastids of the cigarette tobacco, Nicotiana tabacum . Transplastomic N. tabacum plants accumulated astaxanthin to up to 1% of the plants' dry weight. We then used grafting, a procedure recently shown to facilitate horizontal genome transfer between plants [5–7], to let the transgenic chloroplast genome move across the graft junction from N. tabacum plants into plants of the nicotine-free tree species Nicotiana glauca . Transplastomic N. glauca trees expressing the synthetic pathway were recovered at high frequency, thus providing a straightforward method for extension of the transplastomic technology to new species. |
| Databáze: | OpenAIRE |
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