Evidence of bovine immunodeficiency virus (BIV) infection: Serological survey in Argentina
| Autor: | Ester Teresa González, María Licursi, Chris Venables, Eduardo Mortola, Estela Bonzo, Jean-Pierre Frossard, V. Vila Roza |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Immunodeficiency Virus
Bovine Synthetic antigen Argentina BLV Cattle Diseases Fluorescent Antibody Technique Seroprevalence Enzyme-Linked Immunosorbent Assay Antibodies Viral Polymerase Chain Reaction Serology I-ELISA Prevalence Animals Serologic Tests BIV General Veterinary Bovine leukemia virus biology Ciencias Veterinarias Bovine immunodeficiency virus Provirus biology.organism_classification Virology Immunodiffusion Dairying DNA Viral Lentivirus Infections Cattle Female Nested polymerase chain reaction IFA |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | This is the first report of serological evidence for bovine immunodeficiency virus (BIV) infection in Argentina. The analysis was performed in 589 dairy bovine sera samples, applying indirect enzyme-linked immunosorbent assay (I-ELISA) using a synthetic antigen (transmembrane peptide, TM) and Immunofluorescent assay (IFA). In this study, 9 dairy herds from 4 Argentinian provinces were evaluated and 12% of the animals tested positive for BIV. Seven of the 9 herds tested were BIV seropositive and the percentage of BIV seropositive animals in the herds ranged from 2% to 42%. Direct detection of BIV provirus applying nested PCR was not conclusive. Antibody detection against bovine leukemia virus (BLV) in all sera was also performed applying immunodiffusion (ID) assay and 59% resulted seropositive. Statistical analysis of the results was carried out and possible evidence of association between BIV and BLV infection was considered. Future studies should be performed including local field isolates strains of BIV. Facultad de Ciencias Veterinarias |
| Databáze: | OpenAIRE |
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