Uricase alkaline enzymosomes with enhanced stabilities and anti-hyperuricemia effects induced by favorable microenvironmental changes
| Autor: | Mi Zhang, Yunli Zhou, Jianyong Wu, Huarong Xiong, Jingqing Zhang, Biyue Zhu, Dan He, Xueyuan Hu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Urate Oxidase Bicine Enzyme Therapy 02 engineering and technology Hyperuricemia Alkalies Bioinformatics Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems In vivo Humans chemistry.chemical_classification Liposome Multidisciplinary biology Chemistry Urate oxidase 021001 nanoscience & nanotechnology Catalase Lipids Nanostructures 030104 developmental biology Enzyme Membrane Cellular Microenvironment Liposomes biology.protein Biophysics Uric acid Erratum 0210 nano-technology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Enzyme therapy is an effective strategy to treat diseases. Three strategies were pursued to provide the favorable microenvironments for uricase (UCU) to eventually improve its features: using the right type of buffer to constitute the liquid media where catalyze reactions take place; entrapping UCU inside the selectively permeable lipid vesicle membranes; and entrapping catalase together with UCU inside the membranes. The nanosized alkaline enzymosomes containing UCU/(UCU and catalase) (ESU/ESUC) in bicine buffer had better thermal, hypothermal, acid-base and proteolytic stabilities, in vitro and in vivo kinetic characteristics, and uric acid lowering effects. The favorable microenvironments were conducive to the establishment of the enzymosomes with superior properties. It was the first time that two therapeutic enzymes were simultaneously entrapped into one enzymosome having the right type of buffer to achieve added treatment efficacy. The development of ESU/ESUC in bicine buffer provides valuable tactics in hypouricemic therapy and enzymosomal application. |
| Databáze: | OpenAIRE |
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