Glycation Methods for Bombesin Analogs Containing the (NαHis)Ac chelator for99mTc(CO)3Radiolabeling
| Autor: | Christian Schweinsberg, Peter Bläuenstein, Dirk Tourwé, Veronique Maes, Roger Schibli, P. August Schubiger, Elisa Garcia-Garayoa, Luc Brans, Simone Daepp |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Biodistribution Peptide Biochemistry chemistry.chemical_compound Glycation Glycomimetic Drug Discovery Tumor Cells Cultured Gastrin-releasing peptide receptor Humans Receptor Chelating Agents Pharmacology chemistry.chemical_classification Organic Chemistry Prostatic Neoplasms Bombesin Organotechnetium Compounds Glycopeptide Receptors Bombesin chemistry Isotope Labeling Molecular Medicine Radiopharmaceuticals |
| Zdroj: | Chemical Biology & Drug Design. 72:496-506 |
| ISSN: | 1747-0285 1747-0277 |
| DOI: | 10.1111/j.1747-0285.2008.00727.x |
| Popis: | The overexpression of peptide receptors in a variety of human carcinomas has generated considerable interest in peptide-based radiopharmaceuticals for peptide receptor imaging and peptide receptor radiotherapy. The gastrin-releasing peptide receptor is overexpressed in human prostate-, breast-, colon- and small cell lung carcinoma cells. We have developed metabolically stable (99m)Tc-radiolabeled bombesin ([Cha(13), Nle(14)]BBS(7-14)) analogs, which bind with high affinity to the gastrin-releasing peptide receptors. However, because of their lipophilicity, they showed unfavorable biodistribution with high hepatic accumulation and hepatobiliary excretion. We now report a study of different glycation methods for [Cha(13), Nle(14)]BBS(7-14) analogs to improve their biodistribution profile. Whereas the glycation using the Maillard reaction was problematic, resulting in low yields, selective introduction of the glycomimetic shikimic acid to the side chain of a Lys residue was possible. A chemoselective ligation of alpha-D-glucose to an amino-oxyacetylated [Cha(13), Nle(14)]BBS(7-14) analog could be achieved, but was complicated by the co-elution of starting peptide and glycopeptide. The best procedure consisted of the [1,3]-cycloaddition of N(3)-beta-D-glucose to a propargylglycine-containing [Cha(13), Nle(14)]BBS(7-14) analog, using a catalytic amount of Cu(I)I. All glycated [Cha(13), Nle(14)]BBS(7-14) analogs showed high affinity for the gastrin-releasing peptide receptor and rapid accumulation into PC-3 tumor cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|