Onlay-graft of 3D printed Kagome-structure PCL scaffold incorporated with rhBMP-2 based on hyaluronic acid hydrogel
| Autor: | Bu-Kyu Lee, Min-Soo Ghim, Yongdoo Park, Sang-Hyug Park, Jeong-Kui Ku, Young-Sam Cho, Young-Kyun Kim, Kang-Gon Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Scaffold Reconstructive surgery medicine.medical_specialty 3d printed Bone Regeneration Polyesters Group ii Biomedical Engineering Bone Morphogenetic Protein 2 Bioengineering Bone morphogenetic protein 2 Biomaterials Rats Sprague-Dawley chemistry.chemical_compound Transforming Growth Factor beta Hyaluronic acid medicine Animals Hyaluronic Acid Bone regeneration Tissue Scaffolds Chemistry Hydrogels Recombinant Proteins Rats Polycaprolactone Printing Three-Dimensional Biomedical engineering |
| Zdroj: | Biomedical materials (Bristol, England). 16(5) |
| ISSN: | 1748-605X |
| Popis: | The onlay-graft, one of the most difficult graft conditions, is used for diverse clinical conditions, including plastic and dental surgery. The graft should withstand continuous pressure from overlying tissues and have excellent bone formation capability in a limited bone contact situation. We recently developed a 3D printed Kagome-structured polycaprolactone (PCL) scaffold that has a stronger mechanical property. This study evaluated the clinical feasibility of this scaffold for onlay-graft use. The value of the scaffold containing recombinant human bone morphogenetic protein-2 in a hyaluronate-based hydrogel (rhBMP-2/HA) to enhance bone regeneration was also assessed. 3D-printed Kagome-PCL scaffolds alone (n = 12, group I) or loaded with rhBMP-2/HA (n = 12, group II) were grafted using a rat calvarial onlay-graft model. Following sacrifice at 2, 4, and 8 weeks, all 3D-printed Kagome-PCL scaffolds were accurately positioned and firmly integrated to the recipient bone. Micro-computed tomography and histology analyses revealed a constant height of the scaffolds over time in all animals. New bone grew into the scaffolds in both groups, but with greater volume in group II. These results suggest the promising clinical feasibility of the 3D-printed Kagome-PCL scaffold for onlay-graft use and it could substitute the conventional onlay-graft in the plastic and dental reconstructive surgery in the near future. |
| Databáze: | OpenAIRE |
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