Isolation of human capillary endothelial cells using paramagnetic beads conjugated to anti-PECAM antibodies
| Autor: | Jeremy P. Springhorn |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD31
Platelet Endothelial Cell Adhesion Molecule biology Chemistry Immunomagnetic Separation Abdominal Fat Adipose tissue Endothelial Cells Capillary endothelial cells Conjugated system Molecular biology General Biochemistry Genetics and Molecular Biology Antibodies Platelet Endothelial Cell Adhesion Molecule-1 biology.protein Humans Antibody |
| Zdroj: | Cold Spring Harbor protocols. 2011(5) |
| ISSN: | 1559-6095 |
| Popis: | INTRODUCTIONThe protocol described below outlines a technique to isolate and maintain pure populations of human capillary endothelial cells using an active selection technique that targets the platelet endothelial cell adhesion molecule (PECAM or CD31). Human abdominal wall adipose tissue is used as the source for capillary endothelial cells, which are partially purified before selection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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