Intestinal Tumorigenesis Is Not Affected by Progesterone Signaling in Rodent Models
Autor: | Patrick G. Groothuis, Daniel W. Hommes, Rutger J. Jacobs, Gijs R. van den Brink, Sietse Mosselman, Jarom Heijmans, James C. H. Hardwick, Izak Biemond, Vanesa Muncan, Laura Graven, Antwan G. Ederveen, Eveline S.M. de Jonge-Muller |
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Přispěvatelé: | Luk, John, Tytgat Institute for Liver and Intestinal Research, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Gastroenterology and Hepatology, Amsterdam institute for Infection and Immunity, Faculteit der Geneeskunde |
Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Mouse
estrogen plus progestin colon-cancer medroxyprogesterone acetate colorectal-cancer postmenopausal women tumor progression receptor knockout mammary-gland mouse models mice lcsh:Medicine Small Cell Transformation chemistry.chemical_compound Mice Intestinal mucosa Receptors Gastrointestinal Cancers Basic Cancer Research Intestine Small Intestinal Mucosa lcsh:Science Progesterone Cancer Multidisciplinary Intestinal Polyposis Animal Models Intestinal epithelium Immunohistochemistry Intestine Colo-Rectal Cancer medicine.anatomical_structure Cell Transformation Neoplastic Oncology Medicine Signal transduction Receptors Progesterone Aberrant crypt foci Research Article Signal Transduction medicine.medical_specialty General Science & Technology medicine.drug_class Colon Mesenchyme Gastroenterology and Hepatology Biology Model Organisms Internal medicine Progesterone receptor Intestinal Neoplasms medicine Animals Cell Proliferation Neoplastic Animal Azoxymethane Prevention lcsh:R Correction Rats Disease Models Animal Endocrinology chemistry Disease Models lcsh:Q Progestins Digestive Diseases Progestin |
Zdroj: | PLoS ONE PloS one, vol 6, iss 7 PLoS ONE, 6(7). Public Library of Science PLoS ONE, Vol 6, Iss 7, p e22620 (2011) PLoS ONE, 6(7):e22620. Public Library of Science PLoS ONE, 6(7) |
ISSN: | 1932-6203 |
Popis: | Clinical data suggest that progestins have chemopreventive properties in the development of colorectal cancer. We set out to examine a potential protective effect of progestins and progesterone signaling on colon cancer development. In normal and neoplastic intestinal tissue, we found that the progesterone receptor (PR) is not expressed. Expression was confined to sporadic mesenchymal cells. To analyze the influence of systemic progesterone receptor signaling, we crossed mice that lacked the progesterone receptor (PRKO) to the Apc(Min/+) mouse, a model for spontaneous intestinal polyposis. PRKO-Apc(Min/+) mice exhibited no change in polyp number, size or localization compared to Apc(Min/+). To examine effects of progestins on the intestinal epithelium that are independent of the PR, we treated mice with MPA. We found no effects of either progesterone or MPA on gross intestinal morphology or epithelial proliferation. Also, in rats treated with MPA, injection with the carcinogen azoxymethane did not result in a difference in the number or size of aberrant crypt foci, a surrogate end-point for adenoma development. We conclude that expression of the progesterone receptor is limited to cells in the intestinal mesenchyme. We did not observe any effect of progesterone receptor signaling or of progestin treatment in rodent models of intestinal tumorigenesis. |
Databáze: | OpenAIRE |
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