Responsiveness of afferent renal nerve units in renovascular hypertension in rats

Autor: Nada Cordasic, Karl F. Hilgers, Kristina Rodionova, Kerstin Amann, Roland E. Schmieder, Roland Veelken, Christian Ott, Johannes Doellner, Salman Rafii-Tabrizi, Mario Schiffer, Tilmann Ditting, Peter Linz, Annalena Karl
Rok vydání: 2021
Předmět:
Zdroj: Pflügers Archiv - European Journal of Physiology. 473:1617-1629
ISSN: 1432-2013
0031-6768
DOI: 10.1007/s00424-021-02591-6
Popis: Previous data suggest that renal afferent nerve activity is increased in hypertension exerting sympathoexcitatory effects. Hence, we wanted to test the hypothesis that in renovascular hypertension, the activity of dorsal root ganglion (DRG) neurons with afferent projections from the kidneys is augmented depending on the degree of intrarenal inflammation. For comparison, a nonhypertensive model of mesangioproliferative nephritis was investigated. Renovascular hypertension (2-kidney, 1-clip [2K1C]) was induced by unilateral clipping of the left renal artery and mesangioproliferative glomerulonephritis (anti-Thy1.1) by IV injection of a 1.75-mg/kg BW OX-7 antibody. Neuronal labeling (dicarbocyanine dye [DiI]) in all rats allowed identification of renal afferent dorsal root ganglion (DRG) neurons. A current clamp was used to characterize neurons as tonic (sustained action potential [AP] firing) or phasic (1–4 AP) upon stimulation by current injection. All kidneys were investigated using standard morphological techniques. DRG neurons exhibited less often tonic response if in vivo axonal input from clipped kidneys was received (30.4% vs. 61.2% control, p p
Databáze: OpenAIRE
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