Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine
| Autor: | Jian Jun Jia, Carl A. Gagnon, Marcelo Gottschalk, Nedzad Music, Maximilien Arella, Faust René Okamba |
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| Přispěvatelé: | Université de Montréal. Faculté de médecine vétérinaire |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Swine
Enzyme-Linked Immunosorbent Assay Replication-defective adenovirus Weight Gain Microbiology Adenoviridae Leukocyte Count Immune system Porcine enzootic pneumonia Mycoplasma hyopneumoniae Immunity Antibody Specificity Animals Adhesins Bacterial Saliva Lung Swine Diseases Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Vaccination Public Health Environmental and Occupational Health Pneumonia of Swine Mycoplasmal biology.organism_classification Acquired immune system Virology Immunity Humoral Bacterial adhesin P97 adhesin Infectious Diseases Immunoglobulin G Bacterial Vaccines biology.protein Molecular Medicine Cytokines Antibody Bronchoalveolar Lavage Fluid |
| Popis: | Mycoplasma hyopneumoniae causes severe economic losses to the swine industry worldwide and the prevention of its related disease, enzootic porcine pneumonia, remains a challenge. The P97 adhesin protein of M. hyopneumoniae should be a good candidate for the development of a subunit vaccine because antibodies produced against P97 could prevent the adhesion of the pathogen to the respiratory epithelial cells in vitro. In the present study, a P97 recombinant replication-defective adenovirus (rAdP97c) subunit vaccine efficiency was evaluated in pigs. The rAdP97c vaccine was found to induce both strong P97 specific humoral and cellular immune responses. The rAdP97c vaccinated pigs developed a lower amount of macroscopic lung lesions (18.5 + or - 9.6%) compared to the unvaccinated and challenged animals (45.8 + or - 11.5%). rAdP97c vaccine reduced significantly the severity of inflammatory response and the amount of M. hyopneumoniae in the respiratory tract. Furthermore, the average daily weight gain was slightly improved in the rAdP97c vaccinated pigs (0.672 + or - 0.068 kg/day) compared to the unvaccinated and challenged animals (0.568 + or - 0.104 kg/day). A bacterin-based commercial vaccine (Suvaxyn MH-one) was more efficient to induce a protective immune response than rAdP97c even if it did not evoke a P97 specific immune response. These results suggest that immunodominant antigens other than P97 adhesin are also important in the induction of a protective immune response and should be taken into account in the future development of M. hyopneumoniae subunit vaccines. |
| Databáze: | OpenAIRE |
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