Effect of K channel blockers on basal and β-agonist stimulated ion transport by fetal distal lung epithelium
| Autor: | Bijan Rafii, Hugh O'Brodovich |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Agonist
medicine.medical_specialty Potassium Channels Physiology medicine.drug_class Terbutaline Sodium Chloride Biology Epithelium Sodium Channels Basal (phylogenetics) chemistry.chemical_compound Pregnancy Physiology (medical) Internal medicine medicine Animals Rats Wistar Lung Cells Cultured Ion transporter Pharmacology Tetraethylammonium Furosemide General Medicine Adrenergic beta-Agonists Rats Amiloride Electrophysiology Pulmonary Alveoli Endocrinology chemistry Barium Quinine Sulfate Female medicine.drug |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 71:54-57 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y93-008 |
| Popis: | To determine whether basolateral K channels play an important role in the basal and β-agonist stimulated ion transport by fetal distal lung epithelium we harvested these cells from fetal rats (20 days gestation, term = 22 days) and studied them in Ussing chambers. Short-circuit current (Isc) fell with basal 3 mM BaCl2 (3.0 ± 0.2 (±SEM) to 2.0 ± 0.2 μA∙cm−2, n = 18, p 2). Basal quinine sulfate (1 mM) also decreased Isc (3.7 ± 0.15 to 3.0 ± 0.10 μA∙cm−2; n = 4, p 2 (3 mM), apical quinine (1 mM), nor bilaterally applied tetraethylammonium (10 mM), lidocaine (1 mM), or 4-aminopyridine (2 mM) decreased Isc. Cell monolayers treated with basal BaCl2 (3 mM) demonstrated an impaired ability to increase their Isc in response to the β2-agonist terbutaline (1 mM). Basal 3 mM BaCl2 also decreased Isc in amiloride (0.1 mM) and furosemide (1 mM) treated monolayers, indicating that barium also affected the previously described amiloride-insensitive Na transport by these cells (n = 8, p 2-stimulated Na transport in fetal distal lung epithelium.Key words: type II alveolar epithelium, potassium channels, β-agonist, sodium transport, Na channels. |
| Databáze: | OpenAIRE |
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